关于阿拉伯糖作为脑透明质酸的组成成分。通过色谱分析、酶分析和化学电离质谱分析加以证实。

On arabinose as a component of brain hyaluronate. Confirmation by chromatographic, enzymatic and chemical ionization-mass spectrometric analyses.

作者信息

Varma R, Vercellotti J R, Varma R S

出版信息

Biochim Biophys Acta. 1977 Apr 27;497(2):608-14. doi: 10.1016/0304-4165(77)90217-3.

Abstract

The controversy about the presence of the pentose arabinose in brain hyaluronate was reinvestigated using modern analytical tehcnics. The purified bovine brain hyaluronate contained the neutral sugars: arabinose, 0.18%; glucose, 0.05%; and fucose, 0.22%. The confirmation of the presence of arabinose was obtained by paper and thin layer chromatography of the neutral sugars in deionized hyaluronate hydrolysate. Gas-liquid chromatography of the aldononitrile peracetate of the pentose isolated by preparative paper chromatography gave a single distinct peak, corresponding to standard arabinose on three columns packed with three different phases. Chemical ionization data and mass spectrum of the aldononitrile peracetate derivative agreed with those of the authentic arabinonitrile tetracetate. Analysis of the isolated pentose with the help of the enzymes L-arabinose isomerase and L-ribulose kinase, which are specific for their substrates, further established its identity as L-arabinose.

摘要

利用现代分析技术，对脑透明质酸中是否存在戊糖阿拉伯糖的争议进行了重新研究。纯化的牛脑透明质酸含有中性糖：阿拉伯糖，0.18%；葡萄糖，0.05%；岩藻糖，0.22%。通过对去离子透明质酸水解产物中的中性糖进行纸色谱和薄层色谱分析，证实了阿拉伯糖的存在。通过制备性纸色谱分离得到的戊糖的全乙酰化醛糖腈的气液色谱分析，在填充有三种不同固定相的三根柱上均给出了一个单一的明显峰，与标准阿拉伯糖相对应。全乙酰化醛糖腈衍生物的化学电离数据和质谱与正宗的四乙酰化阿拉伯糖腈的数据一致。借助对其底物具有特异性的L-阿拉伯糖异构酶和L-核糖ulose激酶对分离得到的戊糖进行分析，进一步确定其为L-阿拉伯糖。