[哮喘儿童蟑螂过敏的研究。蟑螂变应原的阳性率及抗原性]

[Study of cockroach allergy in asthmatic children. The positive rates and antigenicity of cockroach allergen].

作者信息

Onoue Y, Murakami G, Takayanagi M, Iwaya M, Kayahara M, Adachi Y, Matsuno M, Adachi Y

机构信息

Department of Pediatrics, Faculty of Medicine, Toyama Medical and Pharmaceutical University.

出版信息

Arerugi. 1995 Oct;44(10):1207-15.

PMID:8579482

Abstract

The specific IgE antibodies to 4 species of cockroach were measured in sera from 51 asthmatic children 6 to 16 years old by RAST and CAP system. Positive rates of RAST to Periplaneta fuliginosa, Blattela germanica, Periplaneta americana and Periplaneta japonica were 17.6%, 29.4%, 19.6% and 15.7%, respectively and those of CAP system to Periplaneta fuliginosa and Blattela germanica were 15.7%, respectively. Among those with positive RAST to 4 species of cockroach there were significant correlations. Significant correlations were observed also between the RAST titer for the whole bodies and feces of Periplaneta fuliginosa and Blattela germanica and immunoblotting analysis of sera obtained from two cockroach-positive patients revealed common sensitive fraction between whole body and feces. Immediate bronchial response was detected by bronchial provocation test. RAST inhibition study indicated no cross-reacting allergenicity between cockroach and mite. Collectively, cockroach is one of important inhalent allergens in asthmatic children.

摘要

采用放射变应原吸附试验（RAST）和酶联免疫斑点技术（CAP系统）检测了51例6至16岁哮喘儿童血清中针对4种蟑螂的特异性IgE抗体。对黑胸大蠊、德国小蠊、美洲大蠊和日本大蠊的RAST阳性率分别为17.6%、29.4%、19.6%和15.7%，CAP系统对黑胸大蠊和德国小蠊的阳性率均为15.7%。在对4种蟑螂RAST阳性的患者中存在显著相关性。对黑胸大蠊和德国小蠊的整体及粪便的RAST滴度之间也观察到显著相关性，对两名蟑螂阳性患者血清的免疫印迹分析显示，整体与粪便之间存在共同的敏感组分。通过支气管激发试验检测即刻支气管反应。RAST抑制研究表明蟑螂与螨之间无交叉反应性变应原性。总体而言，蟑螂是哮喘儿童重要的吸入性变应原之一。