Turner A S, Mallinckrodt C H, Alvis M R, Bryant H U
Department of Clinical Sciences, Colorado State University, Ft. Collins 80523, USA.
Bone. 1995 Oct;17(4 Suppl):381S-387S. doi: 10.1016/8756-3282(95)00315-5.
As different large animal models of osteopenia and osteoporosis are explored, the use of DXA to rapidly, non-invasively and accurately estimate BMD will become widespread. We used DXA in live sheep and cadaveric material and the areas of trabecular bone that are most accessible on a simple, repeatable basis in the sheep were the lumbar vertebrae (L4-L6/L5-L7), the CAL and the DR. We performed dual-energy X-ray absorptiometry (DXA) using an Hologic QDR 1000-W bone densitometer to measure bone mineral density (BMD) at various regions of interest in anesthetized sheep and cadaveric specimens of sheep. In vivo measurements of L4-L6/L5-L7, the calcaneus (CAL) and distal radius (DR) in 48 intact 3 to 5-year-old ewes (same breed) were performed. Correlations between the different bones were investigated. In an in vivo precision study, BMD of L3-L6/L7, CAL and DR was determined with one animal repositioned between 10 scans of each bone. In another study, ex-vivo BMD measurements of the proximal and distal femur, proximal tibia, and proximal humerus were performed on isolated bones of 45 ewes of similar age. Excised vertebrae were scanned on the Hologic QDR 1000-W and on a Lunar DPX (at another site) and the data were compared. Correlations of BMD between individual vertebrae in anesthetized sheep were excellent (r = 0.944- 0.843; P < 0.0001). Correlation between BMD of individual vertebrae and CAL was good (r = 0.677-0.630), while correlation between BMD of individual vertebrae and DR was also good (r = 0.551-0.507; P < .0001).(ABSTRACT TRUNCATED AT 250 WORDS)
随着不同的骨质疏松和骨质减少大型动物模型被探索，使用双能X线吸收法（DXA）快速、无创且准确地估计骨密度的方法将变得广泛应用。我们在活体绵羊和尸体材料上使用了DXA，在绵羊身上简单、可重复获取的小梁骨区域是腰椎（L4 - L6/L5 - L7）、跟骨（CAL）和桡骨远端（DR）。我们使用Hologic QDR 1000 - W骨密度仪进行双能X线吸收法（DXA），以测量麻醉绵羊和绵羊尸体标本中各个感兴趣区域的骨密度（BMD）。对48只完整的3至5岁母羊（同一品种）进行了L4 - L6/L5 - L7、跟骨（CAL）和桡骨远端（DR）的活体测量。研究了不同骨骼之间的相关性。在一项活体精度研究中，在对每块骨进行10次扫描期间重新放置一只动物，测定L3 - L6/L7、CAL和DR的骨密度。在另一项研究中，对45只年龄相似的母羊的离体骨骼进行了股骨近端和远端、胫骨近端和肱骨近端的骨密度测量。切除的椎骨在Hologic QDR 1000 - W和Lunar DPX（在另一个地点）上进行扫描并比较数据。麻醉绵羊中各个椎体之间的骨密度相关性极佳（r = 0.944 - 0.843；P < 0.0001）。各个椎体的骨密度与跟骨的相关性良好（r = 0.677 - 0.630），而各个椎体的骨密度与桡骨远端的相关性也良好（r = 0.551 - 0.507；P < 0.0001）。（摘要截断于250字）
