Briend E, Colle J H, Fontan E, Saklani-Jusforgues H, Fauve R M
Unité d'Immunophysiologie Cellulaire, Institut Pasteur, Paris, France.
Int Immunol. 1995 Nov;7(11):1753-61. doi: 10.1093/intimm/7.11.1753.
HGP92 has been shown to enhance in vitro and in vivo the bactericidal and tumoricidal activity of mouse macrophages. In this study we investigated the effect of HGP92 on the accumulation of cytokine mRNA in mouse inflammatory, peritoneal macrophages and the monocytic cell line J774. HGP92 significantly enhanced the level of cytokine mRNA for IL-1 alpha, IL-1 beta, IL-6, IL-10, IL-12, TNF-alpha and GM-CSF during the first 24 h of the incubation. This effect triggered by HGP92 was comparable to that obtained with lipopolysaccharide (LPS), which is a strong cytokine inducer. This accumulation of cytokine mRNA in macrophages was correlated with secretion of IL-6 and TNF-alpha in cell supernatant. The release of IL-6 was HGP92 concentration dependent over a range of 0.3-10 micrograms/ml with a maximum production obtained after a 24 h incubation of inflammatory macrophages with HGP92. This effect was shown not to be due to contamination of HGP92 by LPS since inflammatory macrophages from C57BL/6 mice were responsive to HGP92 pretreated with polymyxin B sulfate and unresponsive to heated HGP92. Stimulating activity of HGP92 was confirmed using macrophages from C3H/HeJ mice. These results suggest that HGP92 might modulate the immune responses by increasing cytokine production by macrophages.
已证明HGP92在体外和体内均可增强小鼠巨噬细胞的杀菌和杀肿瘤活性。在本研究中，我们调查了HGP92对小鼠炎性腹腔巨噬细胞和单核细胞系J774中细胞因子mRNA积累的影响。在孵育的最初24小时内，HGP92显著提高了IL-1α、IL-1β、IL-6、IL-10、IL-12、TNF-α和GM-CSF的细胞因子mRNA水平。HGP92引发的这种效应与脂多糖（LPS，一种强大的细胞因子诱导剂）所产生的效应相当。巨噬细胞中细胞因子mRNA的这种积累与细胞上清液中IL-6和TNF-α的分泌相关。在0.3-10微克/毫升的范围内，IL-6的释放呈HGP92浓度依赖性，炎性巨噬细胞与HGP92孵育24小时后可获得最大产量。已证明这种效应并非由于HGP92被LPS污染所致，因为C57BL/6小鼠的炎性巨噬细胞对用硫酸多粘菌素B预处理的HGP92有反应，而对加热的HGP92无反应。使用C3H/HeJ小鼠的巨噬细胞证实了HGP92的刺激活性。这些结果表明，HGP92可能通过增加巨噬细胞产生细胞因子来调节免疫反应。
