Johansson C B, Morberg P
University of Gothenburg, Department of Handicap Research, Göteborg, Sweden.
Biomaterials. 1995 Sep;16(13):1037-9. doi: 10.1016/0142-9612(95)94913-6.
Ten commercially pure titanium implants were inserted in the tibia of ten mature New Zealand white rabbits for a healing period of 3 months. Cut and ground sections were performed on transversely and longitudinally cut tibia bone with implants in situ. Each implant with surrounding bone was processed by (a) dividing it into two parts by cutting transversely through the tibia and then (b) cutting one of the implant halves longitudinally through the tibia. In both cases 10 microns sections were made. Computerized histomorphometrical calculations of the bone-to-metal contact and the bone area in the threads were performed and comparisons of the differently cut sections were made for the same implant. Larger amounts of bone-to-metal contact and bone area in the threads were observed in the longitudinally cut samples in comparison to transversely cut ones. A strong statistically significant difference was obtained when comparing the transversely with the longitudinally cut samples with respect to bony contacts in the cortical region, demonstrating 25% more bone-to-metal contact lengths in the longitudinal cutting direction of the tibia. Quantitative histomorphometrical comparisons should, therefore, be performed on samples that are cut in the same direction.
将10枚商业纯钛种植体植入10只成年新西兰白兔的胫骨中，愈合期为3个月。对带有原位种植体的胫骨进行横向和纵向切割，制作切片并研磨。将每个带有周围骨组织的种植体进行如下处理：(a) 通过横向切割胫骨将其分成两部分，然后(b) 将其中一半种植体沿胫骨纵向切割。两种情况下均制作10微米厚的切片。对骨与金属的接触以及螺纹中的骨面积进行计算机化组织形态计量学计算，并对同一种植体的不同切割切片进行比较。与横向切割的样本相比，纵向切割的样本中观察到更多的骨与金属接触以及螺纹中的骨面积。在比较横向和纵向切割的样本在皮质区域的骨接触时，获得了具有统计学意义的显著差异，表明在胫骨纵向切割方向上骨与金属的接触长度多25%。因此，应在沿相同方向切割的样本上进行定量组织形态计量学比较。
