Oldenburg H S, Pruitt J H, Lazarus D D, Rogy M A, Chizzonite R, Lowry S F, Moldawer L L
Department of Surgery, Cornell University Medical College, New York, New York 10021, USA.
Cytokine. 1995 Aug;7(6):510-6. doi: 10.1006/cyto.1995.0069.
Neutralizing monoclonal antibodies against the murine interleukin 1 (IL-1) type I (mAb 35F5) and type II receptor (mAb 4E2) were used to passively immunize mice prior to exogenous murine IL-1 alpha administration or a sterile-turpentine induced abscess. When mice were passively immunized with 35F5, the anorexia, weight loss and increased plasma acute phase protein levels in response to exogenous IL-1 alpha administration or a turpentine abscess were significantly attenuated. In contrast, passive immunization with 4E2 had only variable effects on food intake, body weight and the hepatic acute phase response in mice administered IL-1 alpha. In mice following a turpentine abscess, type II receptor blockade (4E2) either had no effect, or in some cases, actually increased the plasma IL-6 and acute phase protein responses. We conclude that in response to a turpentine abscess, the anorexia, weight loss and the induction of several hepatic acute phase reactants result in part from IL-1 binding to its type I receptor. Binding of IL-1 to the type II IL-1 receptor does not appear to be involved in the induction of these host nonspecific responses to inflammation.
在给予外源性小鼠白细胞介素1α（IL-1α）或无菌松节油诱导脓肿之前，使用针对小鼠白细胞介素1（IL-1）I型（单克隆抗体35F5）和II型受体（单克隆抗体4E2）的中和单克隆抗体对小鼠进行被动免疫。当用35F5对小鼠进行被动免疫时，对外源性IL-1α给药或松节油脓肿产生的厌食、体重减轻和血浆急性期蛋白水平升高均得到显著减轻。相比之下，用4E2进行被动免疫对给予IL-1α的小鼠的食物摄入量、体重和肝脏急性期反应只有可变的影响。在松节油脓肿后的小鼠中，II型受体阻断（4E2）要么没有效果，要么在某些情况下，实际上会增加血浆IL-6和急性期蛋白反应。我们得出结论，在对松节油脓肿的反应中，厌食、体重减轻和几种肝脏急性期反应物的诱导部分是由于IL-1与其I型受体结合所致。IL-1与II型IL-1受体的结合似乎与宿主对炎症的这些非特异性反应的诱导无关。
