Ruiz M, Ortega A
Departamento de Genética y Biología Molecular, CINVESTAV-IPN, México, DF, México.
Neuroreport. 1995 Oct 23;6(15):2041-4. doi: 10.1097/00001756-199510010-00021.
The properties of a transport system specific for L-glutamate and L-aspartate activity expressed in Bergmann glia cells from chick cerebellum were examined. The uptake of D-[3H]aspartate was inhibited by the endogenous substrates L-aspartate (Ki = 62 microM) and L-glutamate (Ki = 60 microM). Of the identified uptake inhibitors, L-aspartate-beta-hydroxamate (Ki = 900 microM), L-alpha-aminoadipate (Ki = 2000 microM), 4,4'diisothiocyanatos-tilbene-2,2'-disulphonic acid (DIDS) (Ki = 1000 microM) and 4-acetamido-4'-isothiocyanatostilbene-2,2'-disulphonic acid (SITS) (Ki = 100 microM) inhibited D-[3H]aspartate uptake. Northern blot analysis, revealed the expression of a chick homologue of a the rat brain L-glutamate/L-aspartate transporter (GLAST). These data suggest that GLAST may be involved in the regulation of the parallel fibre-Purkinje cell synapse ensheathed by Bergmann glia.
对鸡小脑伯格曼胶质细胞中表达的L-谷氨酸和L-天冬氨酸活性特异性转运系统的特性进行了研究。内源性底物L-天冬氨酸（Ki = 62微摩尔）和L-谷氨酸（Ki = 60微摩尔）抑制了D-[3H]天冬氨酸的摄取。在已鉴定的摄取抑制剂中，L-天冬氨酸-β-异羟肟酸（Ki = 900微摩尔）、L-α-氨基己二酸（Ki = 2000微摩尔）、4,4'-二异硫氰酸根合芪-2,2'-二磺酸（DIDS）（Ki = 1000微摩尔）和4-乙酰氨基-4'-异硫氰酸根合芪-2,2'-二磺酸（SITS）（Ki = 100微摩尔）抑制了D-[3H]天冬氨酸的摄取。Northern印迹分析显示大鼠脑L-谷氨酸/L-天冬氨酸转运体（GLAST）的鸡同源物的表达。这些数据表明，GLAST可能参与由伯格曼胶质细胞包裹的平行纤维-浦肯野细胞突触的调节。
