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Isolation and characterization of Locusta migratoria accessory gland myotropin I (Lom-Ag-MT-I) from the brain of the Colorado potato beetle, Leptinotarsa decemlineata.

作者信息

Spittaels K, Vankeerberghen A, Schoofs L, Proost P, Van Damme J, De Loof A

机构信息

Zoologisch Instituut, Katholieke Universiteit Leuven, Belgium.

出版信息

Arch Insect Biochem Physiol. 1996;31(2):149-55. doi: 10.1002/(SICI)1520-6327(1996)31:2<149::AID-ARCH3>3.0.CO;2-V.

DOI:10.1002/(SICI)1520-6327(1996)31:2<149::AID-ARCH3>3.0.CO;2-V
PMID:8580496
Abstract

A novel myotropic Colorado potato beetle peptide, active in the Locusta oviduct motility assay, was isolated from a methanolic extract of 9,000 brain complexes of adult Leptinotarsa decemlineata by means of HPLC. Its sequence is Gly-Phe-Lys-Asn-Val-Ala-Leu-Ser-Thr-Ala-Arg-Gly-Phe-NH2. This peptide is identical to Lom-AG-MT-I, a myotropin previously isolated from the male accessory glands of Locusta migratoria, using the L. migratoria oviduct motility bioassay as a monitoring system. It strongly stimulated the frequency, amplitude, and tonus of the myogenic oviduct contractions, even at low concentrations.

摘要

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