Cazaux C, Sterin-Borda L, Gorelik G, Cremaschi G
Centro de Estudios Farmacológicos y Botánicos (CEFYBO)-CONICET, Buenos Aires.
Acta Physiol Pharmacol Ther Latinoam. 1995;45(1):43-8.
beta adrenergic receptors (R) on murine lymphocytes stimulated with concanavalin A (Con A) and tumor lymphoid cell line (BW5147) were characterized by beta radioligand binding of 125Icyanopindolol (125I-CYP). The functionality was also assesses by hormone-dependent changes in cyclic 3', 5', adenosine monophosphate (cAMP) levels on intact cell. Both types of cells displayed a reduced number of beta adrenergic receptors by 125I-CYP specific binding. However BW5147 cell line were unable to respond to beta agonist stimulation meanwhile the response of lymphocytes stimulated with Con A was lower than normal lymphocytes. Despite this difference both kind of cells displayed an increase in cAMP production induced by Prostaglandin E1 (PGE1). It can be concluded that functional beta R are absent in BW5147 cell line. The possible implication of alternative transmission pathway and neuroendocrine control in tumor lymphoid cells is discussed.
用刀豆球蛋白A（Con A）和肿瘤淋巴细胞系（BW5147）刺激的小鼠淋巴细胞上的β肾上腺素能受体（R），通过125I氰胍心安（125I-CYP）的β放射性配体结合进行表征。其功能还通过完整细胞上依赖激素的环3',5'-腺苷单磷酸（cAMP）水平变化来评估。两种类型的细胞通过125I-CYP特异性结合显示β肾上腺素能受体数量减少。然而，BW5147细胞系对β激动剂刺激无反应，而Con A刺激的淋巴细胞的反应低于正常淋巴细胞。尽管存在这种差异，但两种细胞经前列腺素E1（PGE1）诱导的cAMP产生均增加。可以得出结论，BW5147细胞系中不存在功能性β受体。讨论了肿瘤淋巴细胞中替代传导途径和神经内分泌控制的可能意义。
