Kaake R H, Crawford D L, Crawford R L
Department of Microbiology, Molecular Biology, and Biochemistry, University of Idaho, Moscow 83844, USA.
Biodegradation. 1995;6(4):329-37. doi: 10.1007/BF00695263.
The degradation pathway for dinoseb (2-sec-butyl-4,6-dinitrophenol) under reducing conditions was investigated. Cultures were inoculated with a dinoseb-degrading anaerobic enrichment culture used in field studies. Biotransformation intermediates were extracted with ethyl acetate and analyzed by high pressure liquid chromatography, gas chromatography, and mass spectrometry. Dinoseb degradation involves reduction of the nitro groups to amino groups followed by replacement with hydroxyl groups. Depending on the pH and redox potential in the culture, these intermediates may exist as quinones or hydroquinones.
研究了在还原条件下地乐酚（2-仲丁基-4,6-二硝基苯酚）的降解途径。用在田间研究中使用的降解地乐酚的厌氧富集培养物接种培养物。用乙酸乙酯提取生物转化中间体，并通过高压液相色谱、气相色谱和质谱进行分析。地乐酚降解涉及硝基还原为氨基，随后被羟基取代。根据培养物中的pH值和氧化还原电位，这些中间体可能以醌或对苯二酚的形式存在。
