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在体外受精-胚胎移植的卵母细胞取出前后,经阴道超声探头套的穿孔率很高。

High rates of perforation are found in endovaginal ultrasound probe covers before and after oocyte retrieval for in vitro fertilization-embryo transfer.

作者信息

Hignett M, Claman P

机构信息

Department of Obstetrics and Gynaecology, Ottawa Civic Hospital, University of Ottawa, Ontario, Canada.

出版信息

J Assist Reprod Genet. 1995 Oct;12(9):606-9. doi: 10.1007/BF02212583.

DOI:10.1007/BF02212583
PMID:8580658
Abstract

OBJECTIVES

To study perforation rates of sterile transvaginal ultrasound probe covers before and after oocyte retrieval (OPU) in an IVF-ET program.

METHODS

Transvaginal ultrasound probe sheaths from two different manufacturers were studied, Cook Innoray (Cook-Canada #TTUPS-100) and Swemed Lab (Frolunda Sweden #715). After controlled ovarian stimulation, OPU was done using the needle guide of a sterile sheathed 5-MHz transvaginal ultrasound transducer (ATL Bothell, Washington, USA). A newer designed Cook probe cover supplied by the manufacturer was also tested after the company was made aware of our initial perforation results. Following each OPU, probe covers were examined for perforations by filling them with water and checking for leaks. If perforations were found, the vaginal transducer was disinfected by soaking for 20 min in 2% gluteraldehyde (Formac). Twenty unused sterile probe covers from each manufacturer were also tested for perforations.

RESULTS

After OPU we found 10/13 (75%) old Cook, and 35/43 (81%) Swemed probe covers to be perforated (NS). Only 5/20 (25%) of the new design Cook probe covers were perforated post OPU (p = 0.000005). Analysis of unused probe covers revealed 13/20 (65%) Cook, and 5/20 (25%) Swemed probe covers to be perforated (P = 0.02). None of 10 new design unused Cook probe covers were perforated before use.

摘要

目的

研究体外受精-胚胎移植(IVF-ET)程序中取卵(OPU)前后无菌经阴道超声探头套的穿孔率。

方法

对来自两个不同制造商的经阴道超声探头护套进行研究,分别是库克英诺瑞(加拿大库克公司#TTUPS-100)和斯韦姆实验室(瑞典弗罗伦达#715)。在控制性卵巢刺激后,使用无菌护套的5兆赫经阴道超声换能器(美国华盛顿州博塞尔的ATL公司)的针引导器进行取卵。在制造商得知我们最初的穿孔结果后,还对其提供的一种新设计的库克探头套进行了测试。每次取卵后,通过向探头套中注水并检查是否漏水来检查探头套是否穿孔。如果发现穿孔,将阴道换能器浸泡在2%的戊二醛(福尔马克)中20分钟进行消毒。还对每个制造商的20个未使用的无菌探头套进行了穿孔测试。

结果

取卵后,我们发现13个旧库克探头套中有10个(75%)、43个斯韦姆探头套中有35个(81%)穿孔(无显著性差异)。新设计的库克探头套在取卵后只有5/20(25%)穿孔(p = 0.000005)。对未使用的探头套分析显示,20个库克探头套中有13个(65%)、20个斯韦姆探头套中有5个(25%)穿孔(P = 0.02)。10个新设计的未使用库克探头套在使用前均未穿孔。

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