拟南芥钙调蛋白cDNA的5'非翻译区是一个包含开放阅读框的独立cDNA。

The 5' untranslated region of Arabidopsis thaliana calmodulin cDNA is an independent cDNA containing an open reading frame.

作者信息

Granot D, Dai N

机构信息

Department of Field Crops and Natural Resources, Institute of Field & Garden Crops, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel.

出版信息

Planta. 1996;198(1):162-3. doi: 10.1007/BF00197600.

DOI:10.1007/BF00197600

PMID:8580768

Abstract

A cDNA clone, pAUK1, with an open reading frame (ORF) coding for a hypothetical 164-amino-acid protein was isolated from an Arabidopsis thaliana (L.) Heynh cDNA library. The clone was attached, tail to tail, to the 3' end of A. thaliana hexokinase cDNA. An almost identical sequence had been previously described as the 5' untranslated region (5' UTR) of A. thaliana calmodulin cDNA (ACaM-2). Sequence comparison with three additional A. thaliana truncated cDNA clones which appear in a database (GenBank) supports the conclusion that pAUK1 is identical to the 5' UTR of ACaM-2 and that the 5' UTR of ACaM-2 is an independent cDNA artificially linked to A. thaliana calmodulin cDNA.

摘要

从拟南芥cDNA文库中分离出一个cDNA克隆pAUK1，其开放阅读框（ORF）编码一种假设的164个氨基酸的蛋白质。该克隆以尾对尾的方式连接到拟南芥己糖激酶cDNA的3'末端。一个几乎相同的序列先前已被描述为拟南芥钙调蛋白cDNA（ACaM - 2）的5'非翻译区（5'UTR）。与数据库（GenBank）中出现的另外三个拟南芥截短cDNA克隆进行序列比较，支持以下结论：pAUK1与ACaM - 2的5'UTR相同，并且ACaM - 2的5'UTR是一个独立的cDNA，它被人工连接到拟南芥钙调蛋白cDNA上。