一种利用四膜虫对磷脂酰链进行¹³C富集的方法。

A method for 13C enrichment of phospholipid acyl chains using Tetrahymena.

作者信息

Nwanze C E, Hanks R, Dodd G, Howarth O

出版信息

Chem Phys Lipids. 1977 Apr;18(3-4):367-73. doi: 10.1016/0009-3084(77)90014-7.

Abstract

A method has been devised for obtaining highly 13C enriched (20-25%) phospholipids and membranes from the ciliate Tetrahymena, the enrichment occurring in the acyl chains. This method provides both phosphatidylcholine and phosphatidylethanolamine with enriched chains. The central feature of the method is the monoaxenic growth of Tetrahymena on an E. coli mutant which has a high incorporation of exogenous acetate into fatty acid chains. The 13C NMR spectra of the phospholipids are sufficiently enriched to permit NMR relaxation studies.

摘要

已设计出一种从纤毛虫四膜虫中获取高度富集13C（20 - 25%）的磷脂和膜的方法，这种富集发生在酰基链中。该方法能提供链中富含13C的磷脂酰胆碱和磷脂酰乙醇胺。该方法的核心特点是让四膜虫在一种大肠杆菌突变体上进行单菌培养生长，这种突变体能将外源乙酸高效掺入脂肪酸链中。所得到的磷脂的13C核磁共振谱的富集程度足以进行核磁共振弛豫研究。