Hamada S, Ogawa M, Okado N
Department of Anatomy, Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Ibaraki, 305 Japan.
Cell Tissue Res. 1995 Dec;282(3):387-97. doi: 10.1007/BF00318871.
Intraspinal serotonin-positive cells and fibers were examined in the chicken lumbar spinal cord following removal of descending serotonin fibers by spinal transection. Co-localization of Leu-enkephalin immunoreactivity in intraspinal serotonin cells was also examined using a double immunofluorescence labeling technique. By one or two weeks after spinal transection, virtually all supraspinal serotonin fibers were eliminated. Intraspinal serotonin cells were located ventral or ventrolateral to the central canal corresponding to laminae VII, VIII, and IX, and the anterior funiculus. Intraspinal serotonin cells sent fibers to (1) the pia mater on the ventral or ventrolateral surface of the spinal cord; (2) vessels in the spinal cord; (3) sympathetic preganglionic column of Terni; (4) other intraspinal serotonin neurons; (5) the central canal. Some 30%-50% of the intraspinal serotonin cells co-localized with Leu-enkephalin. Intraspinal serotonin fibers co-containing Leu-enkephalin were observed in the pia mater located on the most lateral surface of the spinal cord.
通过脊髓横断去除下行5-羟色胺纤维后，对鸡腰段脊髓内的5-羟色胺阳性细胞和纤维进行了检查。还使用双重免疫荧光标记技术检查了5-羟色胺细胞内亮氨酸脑啡肽免疫反应性的共定位。脊髓横断后一到两周，几乎所有的脊髓上5-羟色胺纤维都被消除。脊髓内5-羟色胺细胞位于中央管腹侧或腹外侧，对应于VII、VIII和IX层以及前索。脊髓内5-羟色胺细胞发出纤维至：(1)脊髓腹侧或腹外侧表面的软膜；(2)脊髓内的血管；(3)特尔尼交感神经节前柱；(4)其他脊髓内5-羟色胺神经元；(5)中央管。约30%-50%的脊髓内5-羟色胺细胞与亮氨酸脑啡肽共定位。在脊髓最外侧表面的软膜中观察到含有亮氨酸脑啡肽的脊髓内5-羟色胺纤维。
