用于分离分化的F9胚胎体中细胞类型的新型流式细胞术方法。
Novel flow-cytometric method for separating cell types in differentiated F9 embryoid bodies.
作者信息
Burdsal C A, Pedersen R A, Hyun W C, Latimer J J
机构信息
Laboratory of Radiobiology and Environmental Health, University of California, San Francisco, USA.
出版信息
Cytometry. 1995 Oct 1;21(2):145-52. doi: 10.1002/cyto.990210206.
The differentiation of F9 teratocarcinoma cells mimics the formation of a mouse embryonic tissue, the primitive endoderm. In vitro, small aggregates of F9 cells, termed embryoid bodies, differentiate in response to retinoic acid and develop a surface epithelium that is characterized by the production of alpha-fetoprotein. In the present study, cellular autofluorescence profiles obtained by fluorescence-activated embryoid bodies were composed of a single type of cell. In contrast, retinoic acid-induced embryoid bodies were composed of two cell types: a major population displaying autofluorescence levels similar to those of cells from undifferentiated embryoid bodies and a second population displaying higher autofluorescence. RNA analyses demonstrated that the transcription of alpha-fetoprotein was associated only with the more highly autofluorescent population, indicating that flow cytometry provides a novel mechanism for the separation of undifferentiated cells from differentiated endoderm cells in F9 embryoid bodies.
F9畸胎癌细胞的分化模拟了小鼠胚胎组织原始内胚层的形成。在体外，F9细胞的小聚集体，即类胚体，会响应视黄酸进行分化，并发育出以甲胎蛋白产生为特征的表面上皮。在本研究中，通过荧光激活的类胚体获得的细胞自发荧光谱由单一类型的细胞组成。相比之下，视黄酸诱导的类胚体由两种细胞类型组成：主要群体显示出与未分化类胚体细胞相似的自发荧光水平，第二种群体显示出更高的自发荧光。RNA分析表明，甲胎蛋白的转录仅与自发荧光更强的群体相关，这表明流式细胞术为从F9类胚体中的未分化细胞与分化的内胚层细胞分离提供了一种新机制。
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