Ormerod M G, Titley J C, Imrie P R
Cancer Research Campaign Centre for Cancer Therapeutics, Institute of Cancer Research, Sutton, Surrey, United Kingdom.
Cytometry. 1995 Nov 1;21(3):294-9. doi: 10.1002/cyto.990210310.
The quality of a DNA histogram as recorded in a flow cytometer can often be improved by gating on forward and orthogonal light scatter. This may be helpful when measuring DNA histograms from formalin-fixed, paraffin-embedded material. We report that nuclei from many human tumours scatter more light orthogonally than those from normal stroma. Gating on light scatter enabled the DNA histogram from the tumour to be recorded with reduced contamination from normal cells. In studies of human mammary carcinomas, we have found that this method has the potential to improve the estimation of S phase and the measurement of DNA index (DI) when the DI is close to 1 (near diploid).
通过对前向光散射和正交光散射进行设门，流式细胞仪记录的DNA直方图质量通常能够得到改善。在测量福尔马林固定、石蜡包埋材料的DNA直方图时，这可能会有所帮助。我们报告称，许多人类肿瘤的细胞核比正常基质的细胞核散射更多的正交光。基于光散射设门能够在记录肿瘤DNA直方图时减少正常细胞的污染。在对人类乳腺癌的研究中，我们发现当DNA指数（DI）接近1（近二倍体）时，该方法有潜力改善S期估计和DNA指数测量。
