Methner U, Koch H, Meyer H
Bundesinstitut für gesundheitlichen Verbraucherschutz und Veterinärmedizin, Bakterielle Tierseuchen und Bekämpfung von Zoonosen, Jena.
Dtsch Tierarztl Wochenschr. 1995 Jun;102(6):225-8.
An infection model is shown for testing the efficacy of prophylactic and therapeutic measures against S. typhimurium and S. enteritidis infections of chickens at different ages. By detecting viable counts in caecum and liver or spleen respectively at weekly intervals after administration of salmonellas (crop instillation) the course of salmonella infection can be grasped. Oral application of the salmonella strains used (S. typhimurium and S. enteritidis) to 1-day-old chickens at a dose of 10(2) cfu/bird, to 8-days-old chickens at a dose of 10(9) cfu/bird and to 5-weeks-old chickens at a dose of 10(10) cfu/bird resulted in a characteristic course of infection but not in clinical symptoms or death. By detecting viable counts of the challenge strain in the organs testing the efficacy of a preceding immunization is possible.
展示了一种感染模型,用于测试针对不同年龄鸡的鼠伤寒沙门氏菌和肠炎沙门氏菌感染的预防和治疗措施的效果。通过在给沙门氏菌(嗉囊滴注)后每周分别检测盲肠、肝脏或脾脏中的活菌数,可掌握沙门氏菌感染的进程。将所用的沙门氏菌菌株(鼠伤寒沙门氏菌和肠炎沙门氏菌)以10(2) cfu/只的剂量口服给1日龄鸡,以10(9) cfu/只的剂量口服给8日龄鸡,以10(10) cfu/只的剂量口服给5周龄鸡,会导致特征性的感染进程,但不会出现临床症状或死亡。通过检测器官中攻击菌株的活菌数,可以测试先前免疫的效果。