Hidalgo A, Urban J, Brand A H
Wellcome/CRC Institute, University of Cambridge, UK.
Development. 1995 Nov;121(11):3703-12. doi: 10.1242/dev.121.11.3703.
Glial cells are thought to play a role in growth cone guidance, both in insects and in vertebrates. In the developing central nervous system of the Drosophila embryo, the interface glia form a scaffold prior to the extension of the first pioneer growth cones. Growing axons appear to contact the glial scaffold as the axon tracts are established. We have used a novel technique for targeted cell ablation to kill the interface glia and thus to test their role in establishment of the embryonic axon tracts. We show that ablation of the interface glia early in development leads to a complete loss of the longitudinal axon tracts. Ablation of the glia later in embryonic development results in defects comprising weakening and loss of axon fascicles within the connectives. We conclude that the interface glia are required first for growth cone guidance in the formation of the longitudinal axon tracts in the Drosophila embryo and then either to direct the follower growth cones, or to maintain the longitudinal axon tracts.
人们认为，无论是在昆虫还是脊椎动物中，神经胶质细胞在生长锥导向方面都发挥着作用。在果蝇胚胎发育中的中枢神经系统里，界面神经胶质细胞在第一批先驱生长锥延伸之前就形成了一个支架。随着轴突束的建立，生长中的轴突似乎会与神经胶质支架接触。我们使用了一种用于靶向细胞消融的新技术来杀死界面神经胶质细胞，从而测试它们在胚胎轴突束建立中的作用。我们发现，在发育早期消融界面神经胶质细胞会导致纵向轴突束完全缺失。在胚胎发育后期消融神经胶质细胞会导致包括连接体内轴突束减弱和丧失在内的缺陷。我们得出结论，界面神经胶质细胞首先是果蝇胚胎中纵向轴突束形成时生长锥导向所必需的，然后要么引导跟随生长锥，要么维持纵向轴突束。
