Ehtesham N Z, Bentur J S, Bennett J
International Centre for Genetic Engineering and Biotechnology, New Delhi, India.
Electrophoresis. 1995 Sep;16(9):1762-5. doi: 10.1002/elps.11501601291.
We described multicopy DNA clones isolated from a partial genomic library of Orseolia oryzae, based on reverse genomic hybridization, suitable for studying genetic variation in the Asian rice gall midge and other isomorphic species. Three clones produced monomorphic DNA band patterns between biotypes of O. oryzae but polymorphic patterns were produced between O. oryzae and O. fluvialis, the paspalum midge. These probes detect changes in the repetitive sequence structure between species and constitute the first genetic markers for distinguishing between field isolates of rice gall midge and related species of Orseolia. These will be useful in identifying and perhaps eradicating alternative hosts for this pest, and detecting early-season outbreaks of O. oryzae from light trap collections.
我们描述了从稻瘿蚊部分基因组文库中分离出的多拷贝DNA克隆，该克隆基于反向基因组杂交技术，适用于研究亚洲稻瘿蚊及其他同形物种的遗传变异。三个克隆在稻瘿蚊不同生物型之间产生单态DNA条带模式，但在稻瘿蚊与大黍瘿蚊之间产生多态模式。这些探针可检测物种间重复序列结构的变化，构成了区分稻瘿蚊田间分离株和稻瘿蚊属相关物种的首批遗传标记。这些标记将有助于识别并可能根除该害虫的替代寄主，以及从诱虫灯收集物中检测稻瘿蚊的早期爆发情况。
