Ehtesham N Z, Bentur J S, Bennett J
International Centre for Genetic Engineering and Biotechnology, New Delhi, India.
Gene. 1995 Feb 14;153(2):179-83. doi: 10.1016/0378-1119(94)00769-o.
We have isolated based on reverse genome hybridization, and sequenced a DNA clone, pNZE25, from a partial genomic library of the Asian rice gall midge Orseolia oryzae (Wood-Mason) (O.o.). Clone pNZE25 is highly A+T rich (67%), lacks any open reading frame and does not display homology to sequences in GenBank. Clone pNZE25 detects a 120-bp repeat in the O.o. genome, as seen from the generation of a 120-bp ladder after Southern analysis of HinfI-digested genomic DNA. When used to probe O.o. genomic DNA digested with DraI, HaeIII or AluI, pNZE25 generates individual specific DNA fingerprints on target DNA isolated from gall midge biotypes collected from different parts of India.
我们基于反向基因组杂交进行了分离，并对来自亚洲稻瘿蚊（Orseolia oryzae (Wood-Mason)，简称O.o.）部分基因组文库的一个DNA克隆pNZE25进行了测序。克隆pNZE25富含A+T（67%），缺乏任何开放阅读框，并且与GenBank中的序列没有同源性。从对经HinfI消化的基因组DNA进行Southern分析后产生的120 bp梯状条带可以看出，克隆pNZE25在O.o.基因组中检测到一个120 bp的重复序列。当用pNZE25探测经DraI、HaeIII或AluI消化的O.o.基因组DNA时，它会在从印度不同地区采集的稻瘿蚊生物型分离出的靶DNA上产生各自特异的DNA指纹图谱。
