Bodegas M E, Montuenga L M, Sesma P
Department of Histology and Pathology, University of Navarra, Pamplona, Spain.
Gen Comp Endocrinol. 1995 Nov;100(2):145-61. doi: 10.1006/gcen.1995.1144.
The neuroendocrine cell population of the respiratory system of Rana temporaria has been studied by means of immunocytochemical methods at the light-microscopic level. Isolated or clustered endocrine cells have been found in the epithelium of the buccal cavity, glottis, larynx, and lung. Nine different types of endocrine isolated cell types can be distinguished according to their immunoreactivity to several regulatory peptides [calcitonin, substance P, bombesin, peptide histidine isoleucine (PHI), cholecystokinin (CCK), and endothelin 1] and neuroendocrine markers (7B2, chromogranin, and serotonin). Neuroepithelial bodies are innervated clusters of cells simultaneously immunoreactive for serotonin and 7B2. Nerves and/or neurons have been detected in different regions of the respiratory system using antibodies against protein gene product 9.5, serotonin, calcitonin gene-related peptide (CGRP), substance P, PHI, helodermin, and CCK.
利用免疫细胞化学方法在光学显微镜水平上对林蛙呼吸系统的神经内分泌细胞群体进行了研究。在口腔、声门、喉和肺的上皮中发现了孤立或成簇的内分泌细胞。根据其对几种调节肽[降钙素、P物质、蛙皮素、肽组氨酸异亮氨酸（PHI）、胆囊收缩素（CCK）和内皮素1]和神经内分泌标志物（7B2、嗜铬粒蛋白和5-羟色胺）的免疫反应性，可区分出九种不同类型的孤立内分泌细胞。神经上皮体是对5-羟色胺和7B2同时具有免疫反应性的神经支配细胞簇。使用针对蛋白基因产物9.5、5-羟色胺、降钙素基因相关肽（CGRP）、P物质、PHI、Helodermin和CCK的抗体，在呼吸系统的不同区域检测到了神经和/或神经元。
