高级杂交系，一种用于精细基因定位的实验群体。
Advanced intercross lines, an experimental population for fine genetic mapping.
作者信息
Darvasi A, Soller M
机构信息
Department of Genetics, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Israel.
出版信息
Genetics. 1995 Nov;141(3):1199-207. doi: 10.1093/genetics/141.3.1199.
An advanced intercrossed line (AIL) is an experimental population that can provide more accurate estimates of quantitative trait loci (QTL) map location than conventional mapping populations. An AIL is produced by randomly and sequentially intercrossing a population that initially originated from a cross between two inbred lines or some variant thereof. This provides increasing probability of recombination between any two loci. Consequently, the genetic length of the entire genome is stretched, providing increased mapping resolution. In this way, for example, with the same population size and QTL effect, a 95% confidence interval of QTL map location of 20 cM in the F2 is reduced fivefold after eight additional random mating generations (F10). Simulation results showed that to obtain the anticipated reduction in the confidence interval, breeding population size of the AIL in all generations should comprise an effective number of > or = 100 individuals. It is proposed that AILs derived from crosses between known inbred lines may be a useful resource for fine genetic mapping.
高级杂交系（AIL）是一种实验群体，与传统的作图群体相比，它能够更准确地估计数量性状基因座（QTL）的图谱位置。AIL是通过对最初源自两个近交系或其某种变体杂交的群体进行随机和连续杂交而产生的。这增加了任意两个基因座之间重组的概率。因此，整个基因组的遗传长度得以延长，从而提高了作图分辨率。例如，在相同的群体大小和QTL效应下，经过额外八代随机交配（F10）后，F2中QTL图谱位置20 cM的95%置信区间缩小了五倍。模拟结果表明，为了实现置信区间的预期缩小，AIL在所有世代的育种群体大小应包含有效数量≥100个个体。有人提出，源自已知近交系杂交的AIL可能是精细遗传作图的有用资源。
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