Bucolo C, Spadaro A
Institute of Pharmacology, University of Catania, Italy.
J Pharm Pharmacol. 1995 Sep;47(9):708-12. doi: 10.1111/j.2042-7158.1995.tb06727.x.
This study evaluated the effect of sodium naproxen (a reversible competitive inhibitor of cyclo-oxygenase) and phenylephrine (a mydriatic alpha-adrenergic agent) eye drops in maintaining atropine mydriasis in the rabbit after paracentesis. Moreover, to assess the influence of these treatments on vascular and cellular inflammatory responses in the rabbit eye, several biochemical parameters were considered. Anterior chamber paracentesis significantly reduced atropine-induced mydriasis and a parallel elevation of proteins, polymorphonuclear leucocytes (PMNs), prostaglandin E2 (PGE2) and leukotriene B4 (LTB4) levels in the secondary aqueous humour (obtained 120 min later) was observed. A significant increase in PMNs in the aqueous humour and a parallel increase in myeloperoxidase activity, a measure of PMN infiltration, in the iris-ciliary body were detected. Atropine-induced mydriasis was maintained in rabbits treated with either sodium naproxen or phenylephrine eye drops. However, only in the former group were the inflammatory parameters significantly reduced, with the exception of aqueous LTB4 levels. The inhibition of the protein influx in the aqueous humour and of the miosis produced by sodium naproxen can be related to the high drug levels in the aqueous humour that were effective in inhibiting the cyclo-oxygenase pathway of arachidonic acid metabolism, whereas the effects on PMN infiltration appear to be independent of significant release of the potent chemotactic agent LTB4, synthesized via the 5-lipoxygenase pathway.
本研究评估了萘普生钠（一种环氧化酶的可逆竞争性抑制剂）和去氧肾上腺素（一种散瞳α-肾上腺素能药物）滴眼液对兔前房穿刺后维持阿托品散瞳的效果。此外，为评估这些治疗对兔眼血管和细胞炎症反应的影响，还考虑了几个生化参数。前房穿刺显著降低了阿托品诱导的散瞳作用，并且观察到在穿刺120分钟后获取的继发性房水中蛋白质、多形核白细胞（PMN）、前列腺素E2（PGE2）和白三烯B4（LTB4）水平同时升高。检测到房水中PMN显著增加，并且虹膜睫状体中髓过氧化物酶活性（一种PMN浸润的指标）同时增加。用萘普生钠或去氧肾上腺素滴眼液治疗的兔维持了阿托品诱导的散瞳作用。然而，仅在前一组中炎症参数显著降低，但房水LTB4水平除外。萘普生钠对房水中蛋白质流入和瞳孔缩小的抑制作用可能与房水中高药物水平有关，该水平有效地抑制了花生四烯酸代谢的环氧化酶途径，而对PMN浸润的影响似乎与通过5-脂氧合酶途径合成的强效趋化剂LTB4的显著释放无关。
