用于分离气单胞菌属的另一种细菌学培养基

An alternative bacteriological medium for the isolation of Aeromonas spp.

作者信息

Jenkins J A, Taylor P W

机构信息

United States Department of Interior, Southeastern Fish Cultural Laboratory, Marion, Alabama 36756, USA.

出版信息

J Wildl Dis. 1995 Apr;31(2):272-5. doi: 10.7589/0090-3558-31.2.272.

DOI:10.7589/0090-3558-31.2.272

PMID:8583652

Abstract

Two solid bacteriologic media were compared for cultivating Aeromonas spp. from piscine sources: the Rimler-Shotts (RS) medium and a starch-glutamate-ampicillin-penicillin-based medium (SGAP-10C) used for the recovery of Aeromonas spp. from water samples. The selective and differential capacities of the media were assessed March through October 1992 by recovery rate and phenotype of 99 isolates representing 15 genera of bacteria. Recovery frequency of Aeromonas spp. (n = 62) was similar at 97% on RS and 95% on SGAP-10C. The SGAP-10C medium proved to be more specific than RS toward Aeromonas species (P < or = 0.005). Use of SGAP-10C at 24 C for 48 hr offers a better choice for the laboratory recovery of Aeromonas spp. from clinical fish specimens.

摘要

比较了两种固体细菌培养基用于从鱼类样本中培养气单胞菌属

Rimler-Shotts（RS）培养基和一种基于淀粉-谷氨酸-氨苄青霉素-青霉素的培养基（SGAP-10C），后者用于从水样中分离气单胞菌属。1992年3月至10月，通过99株代表15个细菌属的菌株的回收率和表型评估了这些培养基的选择性和鉴别能力。气单胞菌属（n = 62）在RS上的回收率为97%，在SGAP-10C上为95%，二者相似。SGAP-10C培养基对气单胞菌属的特异性高于RS（P≤0.005）。在24℃下使用SGAP-10C培养48小时，为从临床鱼类标本中实验室分离气单胞菌属提供了更好的选择。