Saruki N
Department of Anesthesiology and Reanimatology, Gunma University School of Medicine, Maebashi.
Masui. 1995 Dec;44(12):1639-47.
Changes in drebrin, MAP2 (postsynaptic marker) and synaptophysin (presynaptic marker) in rat brains were examined after 20 min of transient cerebral ischemia. Immunoreactivity for drebrin and MAP2 in hippocampus CA1 area decreased 7 days after ischemia. The immunoreactivity for debrin in stratum lucidum of hippocampus CA3 area increased 7 days after ischemia. Sodium dodecyl sulfate gel electrophoresis and immunoblot procedures using an antibody to drebrin, MAP2 and synaptophysin were carried out. The levels of drebrin and MAP2 in hippocampus decreased significantly 4 hours and 7 days after recirculation. In contrast, the level of synaptophysin was unchanged. The levels of each protein in cerebral cortex showed no significant changes. The changes after ischemia seemed to occur at the same time both in the dendritic spines and in their shafts, and the increase of the immunoreactivity for drebrin in CA3 might suggest the change of cytoskeletal protein synthesis in survived neurons.
在短暂性脑缺血20分钟后，检测大鼠大脑中肌动蛋白结合蛋白、微管相关蛋白2（突触后标记物）和突触素（突触前标记物）的变化。缺血7天后，海马CA1区肌动蛋白结合蛋白和微管相关蛋白2的免疫反应性降低。缺血7天后，海马CA3区透明层中肌动蛋白结合蛋白的免疫反应性增加。采用十二烷基硫酸钠凝胶电泳和免疫印迹法，使用针对肌动蛋白结合蛋白、微管相关蛋白2和突触素的抗体进行检测。再灌注4小时和7天后，海马中肌动蛋白结合蛋白和微管相关蛋白2的水平显著降低。相比之下，突触素水平未发生变化。大脑皮层中各蛋白水平无显著变化。缺血后的变化似乎在树突棘及其轴突中同时发生，CA3区肌动蛋白结合蛋白免疫反应性的增加可能表明存活神经元中细胞骨架蛋白合成发生了变化。
