Nagai M A, Butugan O, Logullo A, Brentani M M
Departmento de Radiologia, Faculdade de Medicina, Universidade de São Paulo, Brazil.
Laryngoscope. 1996 Feb;106(2 Pt 1):190-5. doi: 10.1097/00005537-199602000-00016.
Biopsies from 25 juvenile nasopharyngeal angiofibromas (JNAs) and respective normal inferior turbinates were examined and compared. The expression patterns of the messenger RNAs (mRNAs) for various growth factors possibly involved in the growth of mesenchymal cells, as well as angiogenesis and fibrosis, were also compared. These growth factors included insulin-like growth factor II (IGF-II), basic fibroblast growth factor (bFGF), vascular endothelial growth factor (VEGF), transforming growth factors-beta1 (TGF-beta1) and platelet-derived growth factors (PDGF-A and PDGF-B). Quantification of mRNA coding for proto-oncogenes and suppressor genes related to proliferation (i.e., c-myc, c-fos, p53) was also undertaken. Tumor and turbinates expressed similar levels of bFGF, VEGF, TGF-beta1, c-myc, c-fos, and PDGF-A mRNAs. The presence of TGF-beta1 protein was confirmed by immunohistochemistry in several structures that characterize the lesions of JNA, which suggests that TGF-beta1 may play a role in the development of the fibrous component of this tumor. PDGF-B and p53 were overexpressed (i.e., twice the mean level found in turbinates) in 50% and 32% of JNAs, respectively but there was no statistical significance when compared with controls. Statistically significant increased expression of IGF-II mRNA was observed in JNA (P = .04). IGF-II mRNA levels were correlated to p53 (P = .05) and PDGF-B (P = .034), indicating a possible synergistic action of such factors in JNA. The results of this study suggest that IGF-II might be a potential growth regulator of nasopharyngeal angiofibromas.
对25例青少年鼻咽血管纤维瘤（JNA）及相应正常下鼻甲进行活检并比较。还比较了可能参与间充质细胞生长、血管生成和纤维化的各种生长因子的信使核糖核酸（mRNA）表达模式。这些生长因子包括胰岛素样生长因子II（IGF-II）、碱性成纤维细胞生长因子（bFGF）、血管内皮生长因子（VEGF）、转化生长因子-β1（TGF-β1）和血小板衍生生长因子（PDGF-A和PDGF-B）。还对与增殖相关的原癌基因和抑癌基因（即c-myc、c-fos、p53）的mRNA进行了定量分析。肿瘤和鼻甲中bFGF、VEGF、TGF-β1、c-myc、c-fos和PDGF-A mRNA表达水平相似。免疫组织化学证实TGF-β1蛋白存在于JNA病变特征性的几种结构中，这表明TGF-β1可能在该肿瘤纤维成分的形成中起作用。分别有50%和32%的JNA中PDGF-B和p53过表达（即鼻甲中平均水平的两倍），但与对照组相比无统计学意义。在JNA中观察到IGF-II mRNA表达有统计学意义的增加（P = 0.04）。IGF-II mRNA水平与p53（P = 0.05）和PDGF-B（P = 0.034）相关，表明这些因子在JNA中可能存在协同作用。本研究结果提示IGF-II可能是鼻咽血管纤维瘤潜在的生长调节因子。
