Department of Otorhinolaryngology, Head and Neck Surgery, Helsinki University Central Hospital, Helsinki, Finland.
Virchows Arch. 2011 Feb;458(2):189-95. doi: 10.1007/s00428-010-1010-9. Epub 2010 Nov 27.
Juvenile nasopharyngeal angiofibroma (JNA) is a highly vascular tumour, occurring almost exclusively in adolescent males. Histogenesis of JNA remains unclear, two optional theories proposing either fibrous or vascular tissue as the tissue of origin. Stem cell-related proteins have been discussed to possibly participate in the growth of these tumours. In our study, we reviewed retrospective clinicopathological data of 26 JNA patients. By immunohistochemistry, we investigated the cellular distribution and expression levels of stem cell-related proteins C-KIT, C-MYC and BMI-1 and their correlation with cell and vessel density of the tumour. Contrary to earlier reports, we detected C-KIT expression in addition to stromal cells also in endothelial cells. The C-KIT expression was more dominant in slit vessels than large vessels. A significant correlation was found between endothelial immunoexpression of C-KIT and cellular density of the tumour. C-MYC and BMI-1 expression was detected in stromal cells only. Due to our finding of C-KIT expression in both stromal and endothelial cells and the strong correlation between the endothelial C-KIT expression and cellular density, we suggest that, besides the stromal tissue, the vascular component might take part in the neoplastic growth of JNA.
青少年鼻咽血管纤维瘤（JNA）是一种高度血管化的肿瘤，几乎仅发生于青少年男性。JNA 的组织发生仍不清楚，有两种可选理论提出其起源组织为纤维组织或血管组织。干细胞相关蛋白被认为可能参与这些肿瘤的生长。在我们的研究中，我们回顾了 26 例 JNA 患者的回顾性临床病理数据。通过免疫组织化学，我们研究了干细胞相关蛋白 C-KIT、C-MYC 和 BMI-1 的细胞分布和表达水平及其与肿瘤细胞和血管密度的相关性。与早期报道相反，我们除了在间质细胞中还检测到内皮细胞中 C-KIT 的表达。在裂孔血管中 C-KIT 的表达比大血管更明显。内皮细胞 C-KIT 免疫表达与肿瘤细胞密度之间存在显著相关性。C-MYC 和 BMI-1 仅在间质细胞中表达。由于我们发现 C-KIT 在间质和内皮细胞中均有表达，并且内皮细胞 C-KIT 表达与细胞密度之间存在很强的相关性，因此我们认为，除了间质组织外，血管成分可能参与 JNA 的肿瘤生长。
