使用细菌和昆虫细胞表达系统纯化His6标签的Rab1蛋白。 - Suppr

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超能文献

Purification of His6-tagged Rab1 proteins using bacterial and insect cell expression systems.

作者信息

Nuoffer C, Peter F, Balch W E

机构信息

Department of Cell Biology, Scripps Research Institute, La Jolla, California 92037, USA.

出版信息

Methods Enzymol. 1995;257:3-9. doi: 10.1016/s0076-6879(95)57003-9.

DOI:10.1016/s0076-6879(95)57003-9

PMID:8583932

Abstract

摘要

相似文献

Purification of His6-tagged Rab1 proteins using bacterial and insect cell expression systems.

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Expression and purification of recombinant His6-tagged guanine nucleotide dissociation inhibitor and formation of its Rab1 complex.

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Expression and purification of mammalian Sarl.

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Expression of Rab9 protein in Escherichia coli: purification and isoprenylation in vitro.

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Use of Rab-GDP dissociation inhibitor for solubilization and delivery of Rab proteins to biological membranes in streptolysin O-permeabilized cells.

Methods Enzymol. 1995;257:243-53. doi: 10.1016/s0076-6879(95)57029-2.

Purification and assay of yeast Sar1p.

Methods Enzymol. 1995;257:41-9. doi: 10.1016/s0076-6879(95)57008-x.

引用本文的文献

Rab1 recruits WHAMM during membrane remodeling but limits actin nucleation.

Mol Biol Cell. 2016 Mar 15;27(6):967-78. doi: 10.1091/mbc.E15-07-0508. Epub 2016 Jan 28.

Molecular basis for Rab prenylation.

J Cell Biol. 2000 Jul 10;150(1):89-103. doi: 10.1083/jcb.150.1.89.

Inhibition of endosome fusion by wortmannin persists in the presence of activated Rab5.

Mol Biol Cell. 1998 Feb;9(2):323-32. doi: 10.1091/mbc.9.2.323.

Mss4 does not function as an exchange factor for Rab in endoplasmic reticulum to Golgi transport.

Mol Biol Cell. 1997 Jul;8(7):1305-16. doi: 10.1091/mbc.8.7.1305.

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Purification of His6-tagged Rab1 proteins using bacterial and insect cell expression systems.

作者信息

Nuoffer C, Peter F, Balch W E

机构信息

Department of Cell Biology, Scripps Research Institute, La Jolla, California 92037, USA.

出版信息

Methods Enzymol. 1995;257:3-9. doi: 10.1016/s0076-6879(95)57003-9.

DOI:10.1016/s0076-6879(95)57003-9

PMID:8583932

Abstract

摘要

相似文献

Purification of His6-tagged Rab1 proteins using bacterial and insect cell expression systems.

Methods Enzymol. 1995;257:3-9. doi: 10.1016/s0076-6879(95)57003-9.

Expression and purification of recombinant His6-tagged guanine nucleotide dissociation inhibitor and formation of its Rab1 complex.

Methods Enzymol. 1995;257:80-3. doi: 10.1016/s0076-6879(95)57012-8.

Purification of baculovirus-expressed Cdc42Hs.

Methods Enzymol. 1995;256:11-5. doi: 10.1016/0076-6879(95)56004-1.

Purification and properties of Rab3A.

Methods Enzymol. 1995;257:57-70. doi: 10.1016/s0076-6879(95)57010-1.

Interaction of ect2 and Dbl with Rho-related GTPases.

Methods Enzymol. 1995;256:90-8. doi: 10.1016/0076-6879(95)56013-0.

High expression cloning, purification, and assay of Ypt-GTPase-activating proteins.

Methods Enzymol. 1995;257:118-28. doi: 10.1016/s0076-6879(95)57017-9.

Expression and purification of mammalian Sarl.

Methods Enzymol. 1995;257:49-53. doi: 10.1016/s0076-6879(95)57009-8.

Expression of Rab9 protein in Escherichia coli: purification and isoprenylation in vitro.

Methods Enzymol. 1995;257:15-21. doi: 10.1016/s0076-6879(95)57005-5.

Use of Rab-GDP dissociation inhibitor for solubilization and delivery of Rab proteins to biological membranes in streptolysin O-permeabilized cells.

Methods Enzymol. 1995;257:243-53. doi: 10.1016/s0076-6879(95)57029-2.

Purification and assay of yeast Sar1p.

Methods Enzymol. 1995;257:41-9. doi: 10.1016/s0076-6879(95)57008-x.

引用本文的文献

Rab1 recruits WHAMM during membrane remodeling but limits actin nucleation.

Mol Biol Cell. 2016 Mar 15;27(6):967-78. doi: 10.1091/mbc.E15-07-0508. Epub 2016 Jan 28.

Molecular basis for Rab prenylation.

J Cell Biol. 2000 Jul 10;150(1):89-103. doi: 10.1083/jcb.150.1.89.

Inhibition of endosome fusion by wortmannin persists in the presence of activated Rab5.

Mol Biol Cell. 1998 Feb;9(2):323-32. doi: 10.1091/mbc.9.2.323.

Mss4 does not function as an exchange factor for Rab in endoplasmic reticulum to Golgi transport.

Mol Biol Cell. 1997 Jul;8(7):1305-16. doi: 10.1091/mbc.8.7.1305.