al-Attas O S
Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia.
Mol Cell Biochem. 1995 Oct 4;151(1):27-31. doi: 10.1007/BF01076892.
Insulin receptor binding was examined in the microvillous membranes of mid-term (20-22 weeks of gestation, MT) and full-term (FT) placentas from patients with gestational diabetes mellitus (GDM) and in normal pregnant control (N). Mid-term placentas were obtained from patients who have had spontaneous abortion. The maximum per cent specific binding (%SB) in MT placenta for GDM was significantly lower (4.8%) compared with the FT placenta (22%, p < 0.001), while in the N group the maximum per cent specific binding for MT placenta was 14.1% compared with 26% for the FT placenta (p < 0.001). Binding data from FT placenta of well-controlled GDM patients were similar with the FT placenta from N group (22% SB for GDM VS 26% SB for N). Even as there were similarities in the binding characteristics of FT placentas from both groups the placental membrane protein content in the GDM group was lower by 50% compared with the N control (2.5 +/- 0.11 VS 4.8 +/- 0.15 mg protein/g placenta respectively, p < 0.001) suggesting that in the GDM group achieving a tight glycemic control could improve receptor affinities. Data from the competitive binding assay of GDM patients showed that the insulin necessary to achieve 50% inhibition (ID50) was significantly lower in MT compared with the FT placenta (0.9 x 10(-9) M VS 3.8 x 10(-9) M, p < 0.001) but in the N placenta there was no alteration in the ID50 of MT and FT placentas (3.1 x 10(-9) M VS 4 x 10(-9) M, p < 0.01, respectively). The present study demonstrated that in GDM the placental insulin receptor binding was significantly lower in spontaneously aborted placenta compared with placentas collected at full-term. Furthermore, these data suggest that the objective to achieve a tight glycemic control in GDM patients could optimize insulin receptor function similar to that of a normal pregnancy. Thus a full term placenta from GDM patients under a well managed glycemic control throughout the entire duration of pregnancy would result in an optimum insulin receptor function.
对患有妊娠期糖尿病（GDM）患者的中期（妊娠20 - 22周，MT）和足月（FT）胎盘的微绒毛膜以及正常妊娠对照组（N）的胎盘微绒毛膜进行胰岛素受体结合检测。中期胎盘取自有自然流产史的患者。与足月胎盘（22%，p < 0.001）相比，GDM患者中期胎盘的最大特异性结合百分比（%SB）显著降低（4.8%），而在N组中，中期胎盘的最大特异性结合百分比为14.1%，足月胎盘为26%（p < 0.001）。血糖控制良好的GDM患者足月胎盘的结合数据与N组的足月胎盘相似（GDM患者为22% SB，N组为26% SB）。尽管两组足月胎盘的结合特征存在相似性，但GDM组的胎盘膜蛋白含量比N组对照低50%（分别为2.5 ± 0.11与4.8 ± 0.15 mg蛋白/克胎盘，p < 0.001），这表明在GDM组实现严格的血糖控制可改善受体亲和力。GDM患者竞争性结合试验的数据显示，与足月胎盘相比，中期胎盘达到50%抑制所需的胰岛素（ID50）显著降低（0.9×10⁻⁹ M对3.8×10⁻⁹ M，p < 0.001），但在N组胎盘中，中期和足月胎盘的ID50没有变化（分别为3.1×10⁻⁹ M对4×10⁻⁹ M，p < 0.01） 。本研究表明，在GDM中，自然流产胎盘的胎盘胰岛素受体结合与足月收集的胎盘相比显著降低。此外，这些数据表明，GDM患者实现严格血糖控制的目标可以优化胰岛素受体功能，类似于正常妊娠。因此，在整个孕期血糖得到良好控制的GDM患者的足月胎盘将导致最佳的胰岛素受体功能。
