Iyer R S, Singh G, Rebello S, Roy S, Bhat R, Vidyasagar D, Gulati A
Department of Pharmaceutics and Pharmacodynamics, University of Illinois at Chicago 60612, USA.
Pharmacology. 1995 Aug;51(2):96-104. doi: 10.1159/000139321.
The concentration of endothelin-1 (ET-1) in the brain regions, heart, and throacic aorta of 1-, 4-, 6- and 8-week-old spontaneously hypertensive (SHR) and normotensive Wistar-Kyoto (WKY) rats was determined using radioimmunoassay. ET-1-like immunoreactivity in the brain regions of 1-week-old WKY and SHR rats was lower compared to older (6 and 8 weeks) rats. ET-1 levels in the central nervous system gradually increased with age in both SHR and WKY rats. However, the concentration of ET-1 in 8-week-old rats was lower in the brain regions of SHR compared to WKY rats. The concentration of ET-1 in the thoracic aorta of SHR (224 +/- 43 pg/g tissue) rats was lower than that of WKY (452 +/- 11 pg/g tissue) rats at 1 week of age. However, ET-1 levels gradually increased with age in SHR rats. By 8 weeks of age, levels of ET-1 in SHR (623 +/- 33 pg/g tissue) rats were higher compared to WKY (439 +/- 62 pg/g tissue) rats. In the heart, ET-1 levels were similar in WKY and SHR rats at 4 weeks of age, but at 8 weeks of age ET-1 levels were higher in SHR rats (364 +/- 33 pg/g tissue) compared to WKY rats (260 +/- 31 pg/g tissue). It appears that at 8 weeks of age when hypertension is fully expressed in rats, ET-1 levels are lower in the central nervous system and are higher in the thoracic aorta and heart of SHR compared to WKY rats.
采用放射免疫分析法测定了1周龄、4周龄、6周龄和8周龄的自发性高血压大鼠（SHR）和正常血压的Wistar-Kyoto大鼠（WKY）脑区、心脏和胸主动脉中内皮素-1（ET-1）的浓度。与年龄较大（6周和8周）的大鼠相比，1周龄WKY和SHR大鼠脑区的ET-1样免疫反应性较低。在SHR和WKY大鼠中，中枢神经系统中的ET-1水平均随年龄逐渐升高。然而，8周龄时，SHR大鼠脑区ET-1的浓度低于WKY大鼠。1周龄时，SHR大鼠（224±43 pg/g组织）胸主动脉中ET-1的浓度低于WKY大鼠（452±11 pg/g组织）。然而，SHR大鼠中ET-1水平随年龄逐渐升高。到8周龄时，SHR大鼠（623±33 pg/g组织）中ET-1的水平高于WKY大鼠（439±62 pg/g组织）。在心脏中，4周龄时WKY和SHR大鼠的ET-1水平相似，但8周龄时，SHR大鼠（364±33 pg/g组织）的ET-1水平高于WKY大鼠（260±31 pg/g组织）。似乎在8周龄大鼠高血压完全表现出来时，与WKY大鼠相比，SHR大鼠中枢神经系统中ET-1水平较低，而胸主动脉和心脏中ET-1水平较高。
