Yoon J Y, Beinfeld M C
Department of Pharmacological and Physiological Science, St. Louis University Medical Center, MO 63104, USA.
Regul Pept. 1995 Oct 20;59(2):221-7. doi: 10.1016/0167-0115(95)00098-v.
The subtilisin-like endoprotease PC1 (PC3) has been implicated in the processing of a number of prohormones. To evaluate whether PC1 may be important for the processing of pro CCK to CCK 8, stable cell lines expressing a portion of the PC1 cDNA in the antisense orientation were established from RIN5F cells. These cells express CCK mRNA, produce and display regulated secretion of CCK 8. One of the clones, R1E8, expresses antisense PC1 mRNA as determined by reverse transcriptase-PCR (RT-PCR) and contains a significantly reduced level of PC1 protein. As compared to both RIN5F and RIN5F control cells (transfected with the expression plasmid containing no antisense message), R1E8 contains only about 30% cell content of CCK 8. These results suggest that PC1 may be important for the processing of CCK 8 from pro CCK.
类枯草杆菌蛋白酶样内切蛋白酶PC1（PC3）与多种激素原的加工处理有关。为了评估PC1对前CCK加工生成CCK 8是否重要，我们从RIN5F细胞中建立了稳定表达反义方向PC1 cDNA片段的细胞系。这些细胞表达CCK mRNA，产生并显示出CCK 8的调节性分泌。其中一个克隆R1E8，通过逆转录-聚合酶链反应（RT-PCR）确定其表达反义PC1 mRNA，并且PC1蛋白水平显著降低。与RIN5F细胞和RIN5F对照细胞（转染了不含反义信息的表达质粒）相比，R1E8细胞中的CCK 8含量仅约为其细胞含量的30%。这些结果表明，PC1可能对前CCK加工生成CCK 8很重要。
