催产素反义核酸可降低压力感受器去神经大鼠的盐摄入量。

Oxytocin antisense reduces salt intake in the baroreceptor-denervated rat.

作者信息

Morris M, Li P, Barrett C, Callahan M F

机构信息

Department of Physiology and Pharmacology, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, NC 27157, USA.

出版信息

Regul Pept. 1995 Oct 20;59(2):261-6. doi: 10.1016/0167-0115(95)00094-r.

DOI:10.1016/0167-0115(95)00094-r

PMID:8584763

Abstract

Experiments were performed to evaluate the role of central oxytocin (OT) in the inhibition of salt intake produced by sinoartic denervation (SAD). The effect of OT antisense treatment on 24 h intake of 2% NaCl in SAD and sham-operated (SO) rats was determined. PVN injection of unmodified antisense oligodeoxynucleotides (ODNs) to OT mRNA decreased intake of 2% NaCl in SAD, but not SO rats. Salt consumption was 22 +/- 4 ml after the injection of control ODN as compared to 8 +/- 4 ml after the OT antisense injection (P < 0.05). SAD animals also demonstrated an increased plasma OT response to salt loading, an elevation from 3.2 +/- 0.7 to 6.9 +/- 0.8 pg/ml. In contrast, salt ingestion produced no significant change in plasma OT in the SO group. The increased endocrine response in the SADs occurred even though salt intake was lower in this group. There were no group differences in plasma electrolytes or posterior pituitary OT content. Results show that OT antisense specifically inhibits salt intake in the denervated rat, suggesting that the central oxytocinergic axis stimulates sodium drive in this experimental model.

摘要

进行实验以评估中枢催产素（OT）在抑制由窦房神经去支配（SAD）产生的盐摄入中的作用。确定了OT反义治疗对SAD和假手术（SO）大鼠24小时2% NaCl摄入量的影响。向室旁核（PVN）注射针对OT mRNA的未修饰反义寡脱氧核苷酸（ODN）可降低SAD大鼠而非SO大鼠的2% NaCl摄入量。注射对照ODN后盐消耗量为22±4 ml，而注射OT反义后为8±4 ml（P<0.05）。SAD动物对盐负荷的血浆OT反应也增加，从3.2±0.7升高至6.9±0.8 pg/ml。相比之下，SO组中盐摄入对血浆OT没有显著影响。尽管该组盐摄入量较低，但SAD中内分泌反应仍增加。血浆电解质或垂体后叶OT含量无组间差异。结果表明，OT反义特异性抑制去神经大鼠的盐摄入，提示中枢催产素能轴在该实验模型中刺激钠驱动力。