Czyzewska A, Mazur L
Department of Animal Physiology, Jagiellonian University, Cracow, Poland.
Teratog Carcinog Mutagen. 1995;15(3):109-14. doi: 10.1002/tcm.1770150303.
The effect of WR-2721 against cyclophosphamide-induced clastogenicity was studied using the in vivo micronucleus assay. The frequency of micronuclei in polychromatic erythrocytes in the peripheral blood of mice treated with WR-2721 and cyclophosphamide (CP), each of the compounds at a dose of 200 mg/kg body weight, was evaluated during the 15-day period. The suppressing effect of WR-2721, given 30 min prior to cyclophosphamide administration, on micronuclei induced by the alkylating agent was demonstrated. The number of micronuclei was increased on day 1 after CP application and declined thereafter with the frequency of micronucleated polychromatic erythrocytes remaining lower in WR-2721 pre-treated mice. The modulatory effect of WR-2721 on the clastogenic activity of cyclophosphamide in the erythropoietic system by the mouse micronucleus test was shown.
使用体内微核试验研究了WR-2721对环磷酰胺诱导的致断裂性的影响。在15天的时间段内，评估了以200mg/kg体重剂量的WR-2721和环磷酰胺（CP）处理的小鼠外周血中多染性红细胞的微核频率。结果表明，在给予环磷酰胺前30分钟给予WR-2721，对烷化剂诱导的微核有抑制作用。环磷酰胺给药后第1天微核数量增加，此后下降，WR-2721预处理的小鼠中多染性红细胞微核化频率保持较低。通过小鼠微核试验显示了WR-2721对环磷酰胺在红细胞生成系统中的致断裂活性的调节作用。
