Nakamura M, Kinjoh K, Miyagi C, Oka U, Sunagawa M, Yamashita S, Kosugi T
Department of Physiology, School of Medicine, University of the Ryukyus, Okinawa, Japan.
Toxicon. 1995 Sep;33(9):1201-6. doi: 10.1016/0041-0101(95)00050-v.
Following the administration of habutobin, the fibrinogen level in the circulating blood of the rabbits decreased. These results showed that the activity of habutobin was retained in vivo. The plasma level of habutobin was determined by a ELISA-double sandwich method. The pharmacokinetics of habutobin from Trimeresurus flavoviridis venom was studied in rabbits following i.v. administration of 50 micrograms kg-1 of habutobin. The time course of the plasma concentration of habutobin fitted a two-compartment open model. The half-life of the distribution phase was 4.43 +/- 1.28 min and that of the elimination phase was 50.42 +/- 7.89 min. The area under the plasma concentration-time curve (AUC) was 38.69 +/- 6.68 micrograms min ml-1. The total body clearance was 3.82 +/- 1.08 ml min-1. When the steady state was reached, the concentration ratio of habutobin in the tissue (Ct) to that in the plasma (Cc), Ct:Cc was 0.47:1. These findings suggest that relatively little habutobin tended to remain in the tissue.
给予矛头蝮蛇毒纤溶酶后，家兔循环血液中的纤维蛋白原水平降低。这些结果表明矛头蝮蛇毒纤溶酶的活性在体内得以保留。采用酶联免疫吸附双抗体夹心法测定矛头蝮蛇毒纤溶酶的血浆水平。静脉注射50微克/千克的矛头蝮蛇毒纤溶酶后，研究了其在兔体内的药代动力学。矛头蝮蛇毒纤溶酶血浆浓度的时间过程符合二室开放模型。分布相半衰期为4.43±1.28分钟，消除相半衰期为50.42±7.89分钟。血浆浓度-时间曲线下面积（AUC）为38.69±6.68微克·分钟/毫升。总体清除率为3.82±1.08毫升/分钟。达到稳态时，矛头蝮蛇毒纤溶酶在组织（Ct）与血浆（Cc）中的浓度比Ct:Cc为0.47:1。这些发现表明相对较少的矛头蝮蛇毒纤溶酶倾向于留在组织中。
