Tokeshi Y, Nakamura M, Kinjoh K, Sunagawa M, Hanashiro K, Kosugi T
1st Department of Physiology, School of Medicine, University of the Ryukyus, Okinawa, Japan.
Toxicon. 1998 Jan;36(1):53-63. doi: 10.1016/s0041-0101(97)00058-5.
This study investigated whether habu antivenom inhibits the clotting activity of habutobin, a thrombin-like enzyme from Trimeresurus flavoviridis venom. Habu antivenom, which is available as a commercial antibody against the crude venom of T. flavoviridis, has been used to treat envenoming by T. flavoviridis (the habu snake). The present study was undertaken to determine whether habu antivenom inhibits the activities of habutobin, which involve digestion of the A alpha chain and release of fibrinopeptide A (FPA) in rabbit fibrinogen. The results of sodium dodecyl sulfate-polyacrylamide gel electrophoresis demonstrated that habu antivenom inhibited the habutobin-induced digestion of the A alpha chain in rabbit fibrinogen. The results of FPA measurements using competitive enzyme-linked immunoassay (CELIA) revealed that habu antivenom inhibited the release of FPA from rabbit fibrinogen induced by habutobin. In addition, a correlation was noted between the digestion of the A alpha chain and release of FPA from rabbit fibrinogen. Analysis of the inhibition kinetics of habu antivenom against the habutobin activity yielded a competitive double-reciprocal plot.
本研究调查了蝮蛇抗毒素是否能抑制黄绿原矛头蝮蛇毒中的类凝血酶——蝮蛇毒素的凝血活性。蝮蛇抗毒素作为一种针对黄绿原矛头蝮蛇粗毒的商业抗体，已被用于治疗黄绿原矛头蝮蛇（蝮蛇）的蛇伤。本研究旨在确定蝮蛇抗毒素是否能抑制蝮蛇毒素的活性，蝮蛇毒素可使兔纤维蛋白原中的Aα链消化并释放纤维蛋白肽A（FPA）。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳结果表明，蝮蛇抗毒素可抑制蝮蛇毒素诱导的兔纤维蛋白原中Aα链的消化。使用竞争性酶联免疫分析（CELIA）进行FPA测量的结果显示，蝮蛇抗毒素可抑制蝮蛇毒素诱导的兔纤维蛋白原中FPA的释放。此外，还发现兔纤维蛋白原中Aα链的消化与FPA的释放之间存在相关性。对蝮蛇抗毒素对蝮蛇毒素活性的抑制动力学分析得出了竞争性双倒数图。
