Tang G Q, Iida T, Yamamoto K, Honda T
Department of Bacterial Infections, Osaka University, Japan.
FEMS Microbiol Lett. 1995 Dec 15;134(2-3):233-8. doi: 10.1111/j.1574-6968.1995.tb07943.x.
The effects of Vibrio parahaemolyticus thermostable direct hemolysis on Intestine 407, a cell line derived from the intestine of human embryos, were investigated. The hemolysin was shown to be cytotoxic to Intestine 407. This cytotoxicity is accompanied by the damage of plasma membrane and lysosomes, as well as cellular degeneration in the form of large transparent blebs. Although an increase in cytosolic free Ca2+ due t the influx of extracellular Ca2+ was observed in cells treated with thermostable direct hemolysin, it was found to be irrelevant to any of the above effects. These results suggest that the effects of thermostable direct hemolysin observed in this study on Intestine 407 are not mediated by Ca(2+)-dependent pathways.
研究了副溶血性弧菌耐热直接溶血素对人胚胎肠道来源的细胞系肠407的影响。结果表明，该溶血素对肠407具有细胞毒性。这种细胞毒性伴随着质膜和溶酶体的损伤，以及细胞以大的透明泡状形式发生变性。尽管在用耐热直接溶血素处理的细胞中观察到由于细胞外Ca2+内流导致胞质游离Ca2+增加，但发现这与上述任何效应均无关。这些结果表明，本研究中观察到的耐热直接溶血素对肠407的作用不是由Ca(2+)依赖性途径介导的。
