Huang X, Wang L, Ding Z, Chen H
Hua Xi Yi Ke Da Xue Xue Bao. 1995 Sep;26(3):271-4.
Adopting the flow chamber, we studied the effect of step increase in shear stress on prostacyclin releasing of human umbilical vein endothelial cell (HUVEC). HUVEC monolayer was exposed to step increase in shear stress levels of 1, 10 and 20 dyne/cm2 for 100 minutes. By radioimmunoassay, 6-keto-PGF1 alpha in flowing out liquid from the flow chamber was measured. The results displayed: when HUVEC was not exposed to shear stress, the PGI2 released from HUVEC was not detectable; when HUVEC was exposed to shear stress, the flow onset led to an initial burst increase of PGI2 production rate, which went down subsequently within several minutes. We noticed that the larger the step increase of shear stress became, the higher the peak value of PGI2 production rate ascended, the quicker the peak value subsequently declined, and the higher the average value of PGI2 releasing rate in 80-100 minutes stood. The results demonstrated that in vivo hemorheological factor-the shear stress of blood flowing stimulated the release of prostacyclin from HUVEC.
采用流动腔，我们研究了剪切应力逐步增加对人脐静脉内皮细胞（HUVEC）释放前列环素的影响。将HUVEC单层暴露于1、10和20达因/平方厘米的剪切应力水平逐步增加的环境中100分钟。通过放射免疫测定法，测量了从流动腔流出液体中的6-酮-PGF1α。结果显示：当HUVEC未暴露于剪切应力时，无法检测到HUVEC释放的PGI2；当HUVEC暴露于剪切应力时，流动开始导致PGI2产生率最初突发增加，随后在几分钟内下降。我们注意到，剪切应力的逐步增加幅度越大，PGI2产生率的峰值上升越高，随后峰值下降越快，并且在80-100分钟内PGI2释放率的平均值越高。结果表明，体内血液流变学因素——血液流动的剪切应力刺激了HUVEC释放前列环素。
