小鼠肌醇多磷酸1-磷酸酶基因的鉴定与染色体定位

Identification and chromosomal mapping of the mouse inositol polyphosphate 1-phosphatase gene.

作者信息

Okabe I, Nussbaum R L

机构信息

Laboratory of Genetic Disease Research, National Institutes of Health, Bethesda, Maryland 20892-4470, USA.

出版信息

Genomics. 1995 Nov 20;30(2):358-60. doi: 10.1006/geno.1995.0030.

DOI:10.1006/geno.1995.0030

PMID:8586440

Abstract

A mouse inositol polyphosphate 1-phosphatase (Inpp1) cDNA fragment (348 bp) was amplified by means of the polymerase chain reaction using a mouse cDNA library as template with primers designed from published human and bovine cDNA sequences. We isolated a 1623-bp full-length Inpp1 cDNA from a mouse brain cDNA library using this amplified cDNA fragment as probe. Amino acid sequences of mouse, human, and bovine inositol polyphosphate 1-phosphatase are highly conserved. Northern blot analysis shows a major transcript of 1.65-kb mRNA and several higher molecular weight mRNAs that are expressed in a variety of mouse tissues. Utilizing the Jackson Lab backcross DNA panel map service, we mapped Inpp1 to chromosome 1, 1.06 cM proximal to Ctla4.

摘要

以小鼠cDNA文库为模板，使用根据已发表的人及牛cDNA序列设计的引物，通过聚合酶链反应扩增出小鼠肌醇多磷酸1 - 磷酸酶（Inpp1）的cDNA片段（348 bp）。我们以该扩增的cDNA片段为探针，从小鼠脑cDNA文库中分离出1623 bp的全长Inpp1 cDNA。小鼠、人及牛肌醇多磷酸1 - 磷酸酶的氨基酸序列高度保守。Northern印迹分析显示，在多种小鼠组织中表达有一个1.65 kb mRNA的主要转录本以及几个分子量更高的mRNA。利用杰克逊实验室回交DNA图谱服务，我们将Inpp1定位到第1号染色体，位于Ctla4近端1.06 cM处。