Jänne P A, Rochelle J M, Martin-DeLeon P A, Stambolian D, Seldin M F, Nussbaum R L
Department of Genetics, University of Pennsylvania, School of Medicine, Philadelphia, USA.
Genomics. 1995 Jul 20;28(2):280-5. doi: 10.1006/geno.1995.1142.
We have determined the chromosomal localization of the murine gene encoding a 75-kDa inositol polyphosphate-5-phosphatase (Inpp5b). Using two independent approaches, fluorescence in situ hybridization and interspecific backcross analysis, we show that Inpp5b maps to distal mouse Chromosome 4. This map position is within the conserved linkage group corresponding to the short arm of human Chromosome 1, where the human homologue, INPP5B, has been shown to map previously. The position of Inpp5b on mouse Chromosome 4 is in the vicinity of the mouse developmental mutation dysgenetic lens (dyl). However, using a genetic approach, we show that Inpp5b maps distal to dyl on mouse Chromosome 4.
我们已经确定了编码75 kDa肌醇多磷酸-5-磷酸酶(Inpp5b)的小鼠基因的染色体定位。通过荧光原位杂交和种间回交分析这两种独立方法,我们发现Inpp5b定位于小鼠4号染色体远端。该图谱位置位于与人类1号染色体短臂相对应的保守连锁群内,之前已证明人类同源基因INPP5B定位于此。Inpp5b在小鼠4号染色体上的位置靠近小鼠发育突变体晶状体发育不全(dyl)。然而,通过遗传学方法,我们发现Inpp5b在小鼠4号染色体上位于dyl的远端。