Shaheen R M, Miseljic S, Doering D L, Wittliff J L
Department of Biochemistry, James Graham Brown Cancer Center, University of Louisville, Kentucky.
J Clin Lab Anal. 1995;9(6):351-8. doi: 10.1002/jcla.1860090604.
Overexpression of cathepsin D in several types of carcinoma in women appears to be associated with a poor clinical course. In this prospective investigation, cathepsin D levels in 170 specimens of normal and neoplastic human tissues were determined simultaneously by enzyme immunoassay (EIA) and immunoradiometric assay (IRMA) to allow comparisons in multicentric studies, such as cooperative clinical trials. Nonmalignant uteri and specially prepared reference powders were also evaluated. Linear regression analysis between the two assays for all specimens [EIA = 0.87(IRMA)-3.18] demonstrated a correlation coefficient (r) of 0.99 (P < 0.001). When malignancies were categorized by the tissue origin (i.e., breast, uterus, ovary, lymph node, and colon), highly significant correlations were also observed (regressions slopes ranged from 0.58 to 1.02). Intra- and interassay controls conducted for the new EIA procedure gave CV% ranging from 4.4 to 10.2, which was similar to the IRMA test for cathepsin D. The results of both assays correlated well and were highly reproducible. Either assay may be used with confidence that comparable cathepsin D values will be obtained in a wide range of tissue biopsies.
组织蛋白酶D在女性多种类型癌症中的过表达似乎与不良临床病程相关。在这项前瞻性研究中，采用酶免疫测定法（EIA）和免疫放射测定法（IRMA）同时检测了170份正常和肿瘤性人体组织标本中的组织蛋白酶D水平，以便在多中心研究（如合作临床试验）中进行比较。还对非恶性子宫组织和特制的参考粉末进行了评估。对所有标本进行的两种检测方法之间的线性回归分析[EIA = 0.87（IRMA）-3.18]显示相关系数（r）为0.99（P < 0.001）。当根据组织来源（即乳腺、子宫、卵巢、淋巴结和结肠）对恶性肿瘤进行分类时，也观察到了高度显著的相关性（回归斜率范围为0.58至1.02）。针对新EIA程序进行的批内和批间对照的变异系数（CV%）范围为4.4至10.2，这与组织蛋白酶D的IRMA检测结果相似。两种检测方法的结果相关性良好且具有高度可重复性。可以放心地使用任何一种检测方法，因为在广泛的组织活检中都能获得相当的组织蛋白酶D值。
