Wang X, Douglas S A, Feuerstein G Z, Ohlstein E H
Department of Cardiovascular Pharmacology, SmithKline Beecham Pharmaceuticals, King of Prussia, Pennsylvania 19406, USA.
J Cardiovasc Pharmacol. 1995;26 Suppl 3:S22-5.
The temporal expressions of endothelin-converting enzyme-1 (ECE-1), preproendothelin-1 (preproET-1), preproET-3, and ETA, ETB receptor mRNAs were studied in rat carotid artery after balloon angioplasty using quantitative reverse transcription and the polymerase chain reaction (RT-PCR). The expression profile of these messages revealed that the increases of ECE-1 mRNA preceded those of preproET-1 and preproET-3. A significant increase in ECE-1 mRNA was observed at 6 h (1.8-fold, p < 0.01) and 24 h (1.7-fold, p < 0.01) after angioplasty, whereas a significant increase in preproET-1 mRNA levels was not observed until day 3 (1.9-fold, p < 0.05) and day 7 (2.1-fold, p < 0.05). A similar delayed increase in preproET-3 mRNA was observed at day 7 (2.8-fold, p < 0.05) and day 14 (2.6-fold, p < 0.05) after angioplasty. The levels of ETA receptor mRNA were elevated 29.3-fold (p < 0.001) and 24.3-fold (p < 0.01) at days 3 and 7 after angioplasty, respectively. The increase in ETB receptor mRNA occurred slightly earlier than that of ETA receptor, showing a 15.1-fold increase at day 1 (p < 0.001) and a 11.3-fold increase at day 3 (p < 0.01) after angioplasty. The data suggest that ECE-1, ET-1, ET-3, and ETA and ETB receptors play an active role in the pathogenesis of neointima formation.
采用定量逆转录聚合酶链反应（RT-PCR），研究了大鼠颈动脉球囊血管成形术后内皮素转换酶-1（ECE-1）、前内皮素原-1（preproET-1）、前内皮素原-3及ETA、ETB受体mRNA的时序表达。这些信息的表达谱显示，ECE-1 mRNA的增加先于前内皮素原-1和前内皮素原-3。血管成形术后6小时（1.8倍，p<0.01）和24小时（1.7倍，p<0.01）观察到ECE-1 mRNA显著增加，而前内皮素原-1 mRNA水平直到第3天（1.9倍，p<0.05）和第7天（2.1倍，p<0.05）才显著增加。血管成形术后第7天（2.8倍，p<0.05）和第14天（2.6倍，p<0.05）观察到前内皮素原-3 mRNA有类似的延迟增加。血管成形术后第3天和第7天，ETA受体mRNA水平分别升高29.3倍（p<0.001）和24.3倍（p<0.01）。ETB受体mRNA的增加比ETA受体稍早出现，血管成形术后第1天增加15.1倍（p<0.001），第3天增加11.3倍（p<0.01）。数据表明，ECE-1、ET-1、ET-3以及ETA和ETB受体在新生内膜形成的发病机制中起积极作用。
