具柄鞭毛金藻（Poteriochromonas stipitata，同物异名：Malham湖赭球藻Ochromonas malhamensis）的囊壳中的几丁质纤维。
Chitinous fibrils in the lorica of the flagellate chrysophyte Poteriochromonas stipitata (syn. Ochromonas malhamensis).
作者信息
Herth W, Kuppel A, Schnepf E
出版信息
J Cell Biol. 1977 May;73(2):311-21. doi: 10.1083/jcb.73.2.311.
Abstract
Ordered microfibrils are formed on the membrane of the cytoplasmic tail of the alga Poteriochromonas after attachment to a substrate. The ultrastructure of native and extracted stalk fibrils was studied with electron microscope methods. In addition, the structural polysaccharide was characterized by hydrolyses, separation of the monomers by thin-layer chromatography, gas-liquid chromatography and amino acid analysis, and by X-ray diffraction. The alkali-resistant fibrils yielded mostly glucosamine upon extensive hydrolysis, and showed X-ray diffraction patterns similar to those of fugal chitin. It is concluded that the resistant core of the fibrils is chitinous.
摘要
藻类波豆虫附着在基质上后，在其细胞质尾膜上形成有序的微原纤维。采用电子显微镜方法研究了天然和提取的柄原纤维的超微结构。此外，通过水解、薄层层析分离单体、气液色谱和氨基酸分析以及X射线衍射对结构多糖进行了表征。耐碱原纤维在广泛水解后主要产生氨基葡萄糖，并显示出与真菌几丁质相似的X射线衍射图谱。得出的结论是，原纤维的抗性核心是几丁质的。
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