Dobrick P, Miksits K, Hahn H
Institut für Medizinische Mikrobiologie und Infektionsimmunologie, Berlin, Federal Republic of Germany.
Mycopathologia. 1995 Sep;131(3):159-66. doi: 10.1007/BF01102895.
An immunohistological study of L3T4(CD4)+ and LYT-2(CD8)+ lymphocytes, Mac-1(CD11b)+ monocytes and granulocytes in experimental murine cryptococcal meningoencephalitis was conducted. To assess the concomitant inflammatory reaction in an extracerebral site, livers were examined in parallel. Mice were infected i.v. with Cryptococcus neoformans, group A/D, and organs were examined immunohistologically for CD4-, CD8- and monocyte- and granulocyte-specific CD11b-phenotypic leukocytes over a period of 60 days. Intracerebrally, agglomerations of cryptococci formed pseudocysts that were surrounded by CD4+ and CD8+ lymphocytes at the end of the second week post-infection, followed by the invasion of monocytes and granulocytes into the lesions. After the fourth week post-infection, most of the invaded lesions were transformed into glious scars. Meningitis was usually marked and showed a homogenous distribution of CD4-, CD8- and CD11b-phenotypic cells, with a predominance of monocytes and CD4+ lymphocytes. Inflammatory infiltrates in the liver were found already 4 days post-infection. CD4+ lymphocytes and monocytes were distributed homogeneously in the infiltrates, with a lower number of CD8+ lymphocytes being located rather in the periphery of the infiltrates. Comparing leukocyte kinetics in brain and liver, an important observation was the delayed immigration of immune cells at the intracerebral cryptococcal lesions as compared with the liver, and the different migration patterns of T-lymphocyte subgroups and macrophages. These results suggest that there are differential leukocyte migration patterns in the liver and brain following disseminated cryptococcosis. The immunological aspects of the observed leukocyte kinetics are discussed.
对实验性小鼠隐球菌性脑膜脑炎中L3T4(CD4)+和LYT - 2(CD8)+淋巴细胞、Mac - 1(CD11b)+单核细胞和粒细胞进行了免疫组织学研究。为评估脑外部位的伴随炎症反应，同时对肝脏进行了检查。小鼠经静脉注射A/D型新型隐球菌，在60天的时间内对器官进行免疫组织学检查，以检测CD4 -、CD8 -以及单核细胞和粒细胞特异性CD11b表型的白细胞。在脑内，感染后第二周结束时，隐球菌聚集形成假囊肿，周围被CD4+和CD8+淋巴细胞包围，随后单核细胞和粒细胞侵入病变部位。感染后第四周后，大多数侵入性病变转化为胶质瘢痕。脑膜炎通常较为明显，CD4 -、CD8 -和CD11b表型细胞呈均匀分布，其中单核细胞和CD4+淋巴细胞占主导。感染后4天在肝脏中发现炎症浸润。CD4+淋巴细胞和单核细胞在浸润物中均匀分布，CD8+淋巴细胞数量较少，主要位于浸润物的周边。比较脑和肝脏中的白细胞动力学，一个重要的观察结果是与肝脏相比，脑内隐球菌病变处免疫细胞的迁移延迟，以及T淋巴细胞亚群和巨噬细胞的迁移模式不同。这些结果表明，播散性隐球菌病后肝脏和脑内存在不同的白细胞迁移模式。本文讨论了所观察到的白细胞动力学的免疫学方面。
