Achidi E A, Perlmann H, Salimonu L S, Asuzu M C, Perlmann P, Berzins K
Department of Chemical Pathology, University College Hospital, Ibadan, Nigeria.
Parasite Immunol. 1995 Oct;17(10):535-40. doi: 10.1111/j.1365-3024.1995.tb00884.x.
Paired maternal-cord serum samples were analysed for antibodies to the Pf155/RESA and circumsporozoite protein (CSP) antigens of Plasmodium falciparum. Malaria parasites were found in 2.6% (3/117) of cord blood and 22.4% (26/116) of maternal samples. Immunofluorescence assays detected P. falciparum-specific IgG antibodies in all paired samples while P. falciparum-specific IgM was detected in 5.8% (7/121) of cord samples. The positivity rates for antibodies to Pf155/RESA and (NANP)6 but not (EENV)6, a C-terminal repeat sequence of Pf155/RESA, were significantly higher in maternal as compared with cord samples. Seropositivity rates to Pf155/RESA and (EENV)6 were not related to maternal parity group while positivity rates to the (NANP)6 peptide were higher in primiparae and multiparae of > or = 4 parity. These data confirm the transplacental transfer of P. falciparum-specific antibodies and the higher incidence of malaria parasitaemia in primiparae. The presence of P. falciparum-specific IgM in some cord samples suggests intrauterine sensitization of the foetus to malarial antigens.
对配对的母血和脐带血血清样本进行分析，检测其中针对恶性疟原虫Pf155/RESA抗原和环子孢子蛋白（CSP）抗原的抗体。在2.6%（3/117）的脐带血样本和22.4%（26/116）的母血样本中发现了疟原虫。免疫荧光检测在所有配对样本中均检测到恶性疟原虫特异性IgG抗体，而在5.8%（7/121）的脐带血样本中检测到恶性疟原虫特异性IgM。与脐带血样本相比，母亲样本中针对Pf155/RESA和（NANP）6而非Pf155/RESA C端重复序列（EENV）6的抗体阳性率显著更高。Pf155/RESA和（EENV）6的血清阳性率与母亲的产次分组无关，而针对（NANP）6肽段的阳性率在初产妇和产次≥4次的经产妇中更高。这些数据证实了恶性疟原虫特异性抗体的胎盘转运以及初产妇中疟疾寄生虫血症的较高发生率。一些脐带血样本中存在恶性疟原虫特异性IgM，提示胎儿在子宫内对疟原虫抗原致敏。
