Wu S C, Blumer J M, Darvill A G, Albersheim P
Department of Biochemistry and Molecular Biology, University of Georgia, Athens 30602-4712, USA.
Plant Physiol. 1996 Jan;110(1):163-70. doi: 10.1104/pp.110.1.163.
A gene (EGL1) encoding an endo-beta-1,4-D-glucanase (EGase, EC 3.2.1.4) of pea (Pisum sativum) has been cloned and characterized. EGL1 encodes a 486-amino acid polypeptide, including a 24-mer putative signal peptide. The mature protein has a calculated molecular mass of 51.3 kD and an isoelectric point of 9.1. This pea EGase shares significant similarity with EGases from other plant species, but it appears to be distinct from the EGases associated with abscission and fruit ripening. Although EGL1 transcripts are detected in all parts of pea plants, they are relatively abundant in flowers and young pods undergoing rapid growth and most abundant in elongating epicotyls of etiolated seedlings. When epicotyl segments (6 mm long, 4 mm from the apical hook) are incubated in a 5 microM solution of the synthetic auxin analog 2,4-dichlorophenoxyacetic acid, the concentration of EGL1 mRNA increases about 10-fold when the segments elongate most rapidly.
编码豌豆（Pisum sativum）内切-β-1,4-D-葡聚糖酶（EGase，EC 3.2.1.4）的基因（EGL1）已被克隆并进行了表征。EGL1编码一个486个氨基酸的多肽，包括一个24个氨基酸的推定信号肽。成熟蛋白的计算分子量为51.3 kD，等电点为9.1。这种豌豆EGase与其他植物物种的EGase具有显著的相似性，但它似乎与与脱落和果实成熟相关的EGase不同。虽然在豌豆植株的所有部位都检测到了EGL1转录本，但它们在快速生长的花和幼荚中相对丰富，在黄化幼苗伸长的上胚轴中最为丰富。当上胚轴切段（6毫米长，距顶端弯钩4毫米）在5 microM的合成生长素类似物2,4-二氯苯氧乙酸溶液中培养时，切段伸长最快时，EGL1 mRNA的浓度增加约10倍。
