Hunt P, Else R W, McConnell I, Hopkins J
Department of Veterinary Pathology, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Summerhall.
Res Vet Sci. 1995 Nov;59(3):201-4. doi: 10.1016/0034-5288(95)90002-0.
Feline CD45 (leucocyte common antigen, LCA) was characterised by using the mouse monoclonal antibody, WC45a. Its specificity was established on the basis of its reactivity with different lymphoid tissues, as judged by flow cytometry and immunohistochemistry, and of the molecular weight of its antigen in different tissues. It reacted with all the leucocyte populations tested including T and B lymphocytes, granulocytes and monocytes. By immunohistology it reacted only with leucocytes and not with endothelia, epithelia or connective tissue. It precipitated cell surface polypeptides, of M(r) 180,000 to 220,000 from lymph node cells, and therefore apparently recognised an epitope represented on most of the isoforms of the CD45 family.
利用小鼠单克隆抗体WC45a对猫CD45（白细胞共同抗原，LCA）进行了表征。通过流式细胞术和免疫组织化学判断，其特异性基于与不同淋巴组织的反应性以及不同组织中其抗原的分子量得以确立。它与所有测试的白细胞群体发生反应，包括T淋巴细胞、B淋巴细胞、粒细胞和单核细胞。通过免疫组织学方法，它仅与白细胞发生反应，而不与内皮细胞、上皮细胞或结缔组织发生反应。它沉淀出淋巴结细胞中分子量为180,000至220,000的细胞表面多肽，因此显然识别了CD45家族大多数异构体上呈现的一个表位。
