Miturski R
Second Clinic of Gynaecological Surgery Medical Academy of Lublin, Poland.
Acta Biochim Pol. 1995;42(2):191-5.
Concentration and electrophoretic pattern of nonhistone chromosomal proteins (NHCP) in cancer and normal endometrium were examined. In normal tissues the NHCP/DNA ratio ranged from 0.6 to 0.8 whereas in malignant tissues from 1.2 to 1.7. Mean nuclear concentration of NHCP was 14.16 pg/cell nucleus of normal endometrium and 33.35 pg/cell nucleus of cancer tissue. The two tissues differed in the electrophoretic pattern. Heavy fractions (> 60 kDa--36%) predominated in normal tissue whereas light fractions (< 30 kDa--48%) in malignant tissue samples. The percentage of intermediate fractions (30-60 kDa) was similar. Neoplastic endometrium shows, irrespective of the patient's age, a high NHCP content. It seems that NHCP, especially the fraction extracted with 0.35 M NaCl, because of its tissue and cell specificity are involved in neoplasm formation.
对癌组织和正常子宫内膜中非组蛋白染色体蛋白（NHCP）的浓度及电泳图谱进行了检测。在正常组织中，NHCP/DNA比值范围为0.6至0.8，而在恶性组织中为1.2至1.7。正常子宫内膜的NHCP平均核浓度为14.16 pg/细胞核，癌组织为33.35 pg/细胞核。两种组织的电泳图谱存在差异。正常组织中重组分（>60 kDa——36%）占主导，而恶性组织样本中轻组分（<30 kDa——48%）占主导。中间组分（30 - 60 kDa）的百分比相似。无论患者年龄如何，肿瘤性子宫内膜均显示出高NHCP含量。似乎NHCP，尤其是用0.35 M NaCl提取的组分，因其组织和细胞特异性而参与肿瘤形成。
