Bujía J, Stammberger M, Holly A, Kastenbauer E
Department of Otolaryngology, Ludwig-Maximilians-University of Munich, Germany.
Am J Otol. 1994 Jul;15(4):532-5.
The stratum corneum (SC) antibodies are present in high titers in the sera of patients who have diseases in which cells containing keratin intermediate filaments have been damaged. Aural cholesteatoma is a skin-related disease of the temporal bone with an extensive production and accumulation of keratinizing epithelium in the middle ear. The aim of the present investigation was to study the humoral immune response to SC in these patients. Sera were obtained from patients with aural cholesteatoma (n = 10) and from normal donors (n = 8) of about the same sex and age distribution. All sera were analyzed for the existence of autoantibodies (IgG and IgM) against SC by both direct and indirect immunofluorescence, using skin and cholesteatoma frozen tissue sections as antigen substrate. The direct immunofluorescence showed a low level of staining intensity for nearly all of the cholesteatoma patients, indicating the in vivo absence of deposits of anti-SC autoantibodies. The indirect immunofluorescence demonstrated positive staining of the SC in both cholesteatoma and skin tissue sections. Furthermore, some patients showed for IgG anti-SC-autoantibodies a positive staining of the highest suprabasal layers of the epithelium of cholesteatoma. IgM anti-SC autoantibodies were always weaker in titer than IgG autoantibodies. Results show that cholesteatoma patients do not have particularly high levels of anti-SC autoantibodies. A humoral response against SC seems not to be a significant event in the pathogenesis of cholesteatoma disease.
在患有细胞内含有角蛋白中间丝的细胞受到损伤的疾病患者血清中，角质层（SC）抗体呈高滴度存在。中耳胆脂瘤是一种与颞骨皮肤相关的疾病，中耳内有大量角质化上皮的产生和积聚。本研究的目的是研究这些患者对SC的体液免疫反应。从患有中耳胆脂瘤的患者（n = 10）和年龄及性别分布大致相同的正常供体（n = 8）中获取血清。使用皮肤和胆脂瘤冰冻组织切片作为抗原底物，通过直接和间接免疫荧光分析所有血清中针对SC的自身抗体（IgG和IgM）的存在情况。直接免疫荧光显示，几乎所有胆脂瘤患者的染色强度都很低，表明体内不存在抗SC自身抗体沉积物。间接免疫荧光显示胆脂瘤和皮肤组织切片中的SC均呈阳性染色。此外，一些患者的IgG抗SC自身抗体在胆脂瘤上皮最高基底层以上呈阳性染色。IgM抗SC自身抗体的滴度始终低于IgG自身抗体。结果表明，胆脂瘤患者的抗SC自身抗体水平并不特别高。针对SC的体液反应似乎在胆脂瘤疾病的发病机制中不是一个重要事件。
