慢生根瘤菌属BR816的共生质粒位点的分离与鉴定：该位点可将窄宿主范围的菜豆共生菌的结瘤能力扩展至银合欢。

Isolation and characterization of a pSym locus of Rhizobium sp. BR816 that extends nodulation ability of narrow host range Phaseolus vulgaris symbionts to Leucaena leucocephala.

作者信息

van Rhijn P, Luyten E, Vlassak K, Vanderleyden J

机构信息

F. A. Janssens Laboratory of Genetics, KU Leuven, Heverlee, Belgium.

出版信息

Mol Plant Microbe Interact. 1996 Jan;9(1):74-7. doi: 10.1094/mpmi-9-0074.

DOI:10.1094/mpmi-9-0074

PMID:8589426

Abstract

Introduction of a cosmid library of megaplasmid DNA of Rhizobium sp. BR816, a broad host range Rhizobium strain, into R. etli CE3, a narrow host range bean symbiont, resulted in the isolation of a transconjugant that could effectively nodulate Leucaena leucocephala. Analysis of the corresponding cosmid, pBRF2, revealed the presence of genes required for elicting nitrogen-fixing nodules on L. leucoephala. Subcloning and Tn5 tagging identified a locus responsible for the host range extension. Sequence analysis of this locus revealed an ORF that shows significant identity with NodO of R. leguminosarum bv. viciae.

摘要

将广宿主范围的根瘤菌菌株中华根瘤菌（Rhizobium sp.）BR816的大质粒DNA的黏粒文库导入宿主范围狭窄的豆类共生体埃氏根瘤菌（R. etli）CE3，得到了一个能有效结瘤银合欢（Leucaena leucocephala）的接合子。对相应黏粒pBRF2的分析表明，其中存在诱导银合欢形成固氮根瘤所需的基因。亚克隆和Tn5标签法确定了一个负责宿主范围扩展的位点。对该位点的序列分析揭示了一个与豌豆根瘤菌（R. leguminosarum bv. viciae）的NodO具有显著同源性的开放阅读框。