Journée-de Korver J G, Oosterhuis J A, Vrensen G F
Department of Ophthalmology, Leiden University Medical Centre, The Netherlands.
Melanoma Res. 1995 Dec;5(6):393-402. doi: 10.1097/00008390-199512000-00002.
The effect of hyperthermia, 50 degrees C applied for 5 min, on the development of lesions in hamster Greene melanomas was investigated. Hyperthermia was induced by a laser that produced radiation at 780-880 nm. Hamster melanomas were also examined after arrest of the blood circulation to differentiate between heat-induced lesions and those caused by ischaemia due to vascular occlusion. Tumours were removed 5 and 30 min and 1, 3, 6 and 24 h after thermotherapy. The cytotoxic effects of heat and ischaemia were examined by light and electron microscopy. Hyperchromatic nuclear staining, the first hyperthermia-induced lesion, was detected in the superficial layers of the tumour 5 min after heat treatment. The lesions had progressed to severe pyknosis and extended into deeper layers of the tumour 1 h after hyperthermia. At 24 h necrosis was observed at a depth of 6 mm. The hyperthermia-induced lesions differed markedly from the ischaemia-induced lesions, in that the latter showed early mitochondrial damage but nuclear pyknosis did not become manifest until 3 h after arrest of the blood circulation.
研究了将50摄氏度高温持续施加5分钟对仓鼠格林黑色素瘤病变发展的影响。高温由产生780 - 880纳米辐射的激光诱导产生。在血液循环停止后，还对仓鼠黑色素瘤进行了检查，以区分热诱导病变和血管阻塞导致的缺血性病变。热疗后5分钟、30分钟以及1、3、6和24小时切除肿瘤。通过光学显微镜和电子显微镜检查热和缺血的细胞毒性作用。热处理5分钟后，在肿瘤表层检测到核染色质深染，这是高温诱导的首个病变。高温1小时后，病变发展为严重的核固缩并延伸至肿瘤更深层。24小时后，在6毫米深处观察到坏死。高温诱导的病变与缺血诱导的病变明显不同，后者早期表现为线粒体损伤，但直到血液循环停止3小时后才出现核固缩。
